Z-D(OMe)E(OMe)VD(OMe)-FMK (Caspase 3/7 Inhibitor)

Z-D(OMe)E(OMe)VD(OMe)-FMK (Caspase 3/7 Inhibitor) Summary

• Immunogen: Z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-FMK
  Z-D(OMe)E(OMe)VD(OMe)-FMK
• Preparation Method: Formula: C30H41O12N4F
  Molecular Weight: 668
  Mass Spec: M+1=669.3.
  Chromatography: EtOAc:9, Hex: 1, Single Spot, Rf:0.5

Applications/Dilutions

• Application Notes: This inhibitor is designed as a methyl ester to facilitate cell permeability. If the intended use is on purified or recombinant enzymes, esterase should be added to generate the free carboxyl groups.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Form: White Solid
  Make a stock solution of 20 mM in high purity DMSO (>99.9%).

Alternate Names for Z-D(OMe)E(OMe)VD(OMe)-FMK (Caspase 3/7 Inhibitor)

• Z-Asp(OMe)-Glu(OMe)-Val-Asp(OMe)-FMK
• Z-D(OMe)E(OMe)VD(OMe)-FMK
• Z-DOMeEOMeVDOMe-FMK

Background

Members of the caspase family play key roles in apoptosis and inflammation. Z-D(OMe)E(OMe)VD(OMe)-FMK (ZDEVD- FMK) is a cell-permeable caspase peptide inhibitor that irreversibly binds to the catalytic site of caspases proteases, and inhibits caspase-mediated apoptosis by preventing caspase activity (reviewed in 1-3). Caspase inhibitors, like Z-DEVD-FMK, with the peptide recognition sequence DEVD are potent inhibitors of caspase-3 and were first described as caspase-3 inhibitors. It is now known that caspase inhibitors with the peptide recognition sequence DEVD also inhibit other caspases, albeit at higher concentrations. However, Z-DEVD-FMK and other inhibitors with the DEVD peptide recognition sequence continue to be denoted as caspase-3 specific inhibitors by a number of sources. The Z-D(OMe)E(OMe)VD(OMe)-FMK (Z-DEVD-FMK) peptide is O-methylated in the P4, P3 and P1 positions providing enhanced stability and increased cell permeability. Z-DEVD-FMK is typically used in assays to inhibit apoptosis. Z-DEVD-FMK has been used in many different types of apoptosis assays and published extensively; for example, a PubMed search reveals over 300 references citing Z-DEVD-FMK between 1999 and 2003. Users may want to consult the literature for additional information regarding applications for Z-DEVD-FMK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.