Yersinia Pestis F1 Antigen Antibody (YPF19)

Product Discontinued

Yersinia Pestis F1 Antigen Antibody (YPF19) Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunised with purified F1 antigen from Y. pestis vaccine strain EV.
• Localization: Capsule
• Specificity: Reacts with capsule-bearing strains of Y. pestis only.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: WB: Predicted molecular weight: 18 kDa.

Reactivity Notes

Reacts with Y. pestis.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Yersinia Pestis F1 Antigen Antibody (YPF19)

• Caf 1
• CAF1
• F1 capsule antigen
• Y. pestis Caf1
• Y. pestis F1 antigen
• Y. pestis F1 capsule antigen
• Yersinia pestis Caf1
• Yersinia pestis F1 capsule antigen

Background

Yersinia pestis is a gram-negative coccobacillus belonging to the Enterobacteriaceae. Yersinia pestis is primarily a rodent pathogen, with humans being an accidental host when bitten by an infected rat flea. The flea draws viable Y. pestis organisms into its intestinal tract. Some Y. pestis in the flea are then regurgitated when the flea gets its blood meal thus transferring the infection to a new host. While growing in the flea, Y. pestis loses its capsular layer, which is made of the F1 (or fraction 1) capsule antigen. Most of the organisms are phagocytosed and killed by the polymorphonuclear leukocytes in the human host. A few bacilli are taken up by tissue macrophages. The macrophages are unable to kill Y. pestis and provide a protected environment for the organisms to synthesize their virulence factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.