Y-79 Nuclear Cell Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Concentration
2 mg/ml

Order Details

Y-79 Nuclear Cell Lysate Summary

Description
Nuclear extract cell lysate (non-denatured)
Localization
retina
Specificity
Y-79 (human retinoblastoma) nuclear extract lysate (non-denatured)
Preparation
Method
Nuclear extract was prepared by using a modified protocol of Dignam et al. Cells were Harvested and homogenized in Buffer A, and then centrifugated at 25,000 g for 20 minutes to remove cytoplasm and pellet the nuclei. The pellet was re-suspended in Buffer C, and then the suspensions were centrifuged to collect nuclear extract. The supernatant was dialyzed against Buffer D. The dialysate was then centrifuged, divided into aliquots, and stored at -80C. The protein concentration was determined by the method of Bradford. The lysate was adjusted to 2 mg/ml.

Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot
Application Notes
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
20mM HEPES pH 7.9, 20%(v/v) glycerol, 50mM KCl, 0.2 mM EDTA, 0.5 mM DTT & 0.5 mM PMSF.
Concentration
2 mg/ml

Lysate Details for Array

Type
Cell
Tissue
Visual
Protein State
Native
Tissue Condition
Retinoblastoma
Subcellular Fraction
Nuclear

Notes

Quality control test: 12.5% SDS-PAGE Stained with Coomassie Blue.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Array L042V4

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