XAGE2 Overexpression Lysate (Native)


Western Blot: XAGE2 Overexpression Lysate (Adult Normal) [NBL1-17898] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XAGE2.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

XAGE2 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC209757 and based on accession number NM_130777. The protein contains a C-terminal DDK tag.
Homo sapiens X antigen family, member 2 (XAGE2), mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
12.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for XAGE2 Overexpression Lysate (Native)

  • Cancer/testis antigen 12.2
  • cancer/testis antigen family 12, member 2
  • CT12.2GAGED3
  • G antigen family D member 3
  • G antigen, family D, 3
  • Protein XAGE-2
  • X antigen family, member 2
  • XAGE-2
  • XAGE2B


This gene is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in normal testis, and in Ewing's sarcoma, rhabdomyosarcoma, a breast cancer and a germ cell tumor. The protein encoded by this gene shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this protein also belongs to a family of CT (cancer-testis) antigens.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB

Publications for XAGE2 Lysate (NBL1-17898) (0)

There are no publications for XAGE2 Lysate (NBL1-17898).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for XAGE2 Lysate (NBL1-17898) (0)

There are no reviews for XAGE2 Lysate (NBL1-17898). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for XAGE2 Lysate (NBL1-17898) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for XAGE2 Lysate (NBL1-17898)

Discover related pathways, diseases and genes to XAGE2 Lysate (NBL1-17898). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on XAGE2

There are no specific blogs for XAGE2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

PAGE1 Antibody
XAGE2 Antibody
XAGE3 Antibody

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our XAGE2 Overexpression Lysate (Native) and receive a gift card or discount.


Gene Symbol XAGE2