XAGE2 Overexpression Lysate (Native)

Images

 
Western Blot: XAGE2 Overexpression Lysate (Adult Normal) [NBL1-17898] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for XAGE2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

XAGE2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209757 and based on accession number NM_130777. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens X antigen family, member 2 (XAGE2), mRNA.
Gene
XAGE2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
12.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for XAGE2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for XAGE2 Overexpression Lysate (Native)

  • Cancer/testis antigen 12.2
  • cancer/testis antigen family 12, member 2
  • CT12.2GAGED3
  • G antigen family D member 3
  • G antigen, family D, 3
  • Protein XAGE-2
  • X antigen family, member 2
  • XAGE-2
  • XAGE2B

Background

This gene is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in normal testis, and in Ewing's sarcoma, rhabdomyosarcoma, a breast cancer and a germ cell tumor. The protein encoded by this gene shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this protein also belongs to a family of CT (cancer-testis) antigens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol XAGE2