Wheat Germ Agglutinin (WGA), Rhodamine

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Wheat Germ Agglutinin (WGA), Rhodamine [RL-1022-10-NB]

Order Details


    • Catalog Number
      RL-1022-10-NB
    • Availability
      Product Discontinued

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Wheat Germ Agglutinin (WGA), Rhodamine Summary

Description
The receptor sugar for WGA is N-acetylglucosamine, with preferential binding to dimers and trimers of this sugar. WGA can bind oligosaccharides containing terminal N-acetylglucosamine or chitobiose, structures which are common to many serum and membrane glycoproteins. Bacterial cell wall peptidoglycans, chitin, cartilage glycosaminoglycans, and glycolipids can also bind WGA. Native WGA has also been reported to interact with some glycoproteins via sialic acid residues (see succinylated WGA).

Rhodamine labeled WGA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 550 nm and the emission maximum is at 575 nm.

Applications/Dilutions

Application Notes
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Packaging, Storage & Formulations

Storage
Store at 4°C.
Buffer
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 25 mM N-acetylglucosamine

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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