VSV-G Epitope Tag Antibody [HRP]

Western Blot: VSV-G Epitope Tag Antibody [HRP] [NB100-2486] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, goat anti-VSV-G antibody used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.

Product Discontinued

VSV-G Epitope Tag Antibody [HRP] Summary

• Immunogen: Synthetic peptide: YTDIEMNRLGK conjugated to KLH.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:100000
  • Immunocytochemistry 1:200-1:500
  • Western Blot 1:1000-1:10000
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS and 0.2% BSA
• Preservative: 0.1% Pro-Clean 400
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VSV-G Epitope Tag Antibody [HRP]

• Vesicular stomatitis virus glycoprotein tag
• VSV epitope tag
• VSV tag
• VSV-G epitope tag
• vsvg tag
• VSVG

Background

The VSV-G epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized VSV-G epitope represents the amino acid sequence YTDIEMNRLGK derived from the Vesicular Stomatitis viral glycoprotein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VSV-G Epitope Tag Antibody (NB100-2486)(1)

Publication using NB100-2486

• Lin F-c, Peng Y, Jones LA et al. Incorporation of CD40 Lig into the Envelope of Pseudotyped Single-Cycle Simian Immunodeficiency Viruses Enhances Immunogenicity. J Virol;83(3):1216-1227. 2009-01-01 [PMID: 19036823]