VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor)

VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor) [NBP2-29335] - VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor) Chemical structure of the ROR gamma T inhibitor VPR-66: C16H21N3O2

VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor) [NBP2-29335] - VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor)VPR-66 inhibition of transcriptional activity determined in CHO cells transfected with ROR gamma.

VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor) [NBP2-29335] - VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor) VPR-66 inhibition of transcriptional activity determined in CHO cells transfected with RORa.

• Concentration: LYOPH

VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor) Summary

• Immunogen: VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor)

Applications/Dilutions

• Application Notes: The ROR gamma T inhibitor is readily soluble in DMSO. This inhibitor should be dissolved in DMSO, ethanol, or other similar organic solvents. The solubility in DMSO exceeds 10 mg/ml. The aqueous solubility is greater than 0.5 mg/ml. Previous studies have used 10 mM stock solutions in DMSO. Prepare concentrated intermediate dilutions in DMSO and then add small volumes to buffer or cell culture media to provide the final aqueous working concentrations. It is not recommended to prepare serial dilutions in aqueous media.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Crystalline, off-white solid; MW = 287.4
• Concentration: LYOPH
• Reconstitution Instructions: Please contact technical support for detailed reconstitution instructions.

Alternate Names for VPR-66, ROR gamma T and ROR alpha Inhibitor (ROR gamma T and ROR alpha Inhibitor)

• VPR 66
• VPR66

Background

h17 cells are a sub-type of T-helper cells that secrete IL-17 and other pro-inflammatory cytokines. Th17 cells have been implicated in driving the pathophysiology of several human autoimmune diseases. Retinoic Acid-Related Orphan Nuclear, Receptors (ROR) are canonical nuclear receptors with transcriptional activity at specific DNA response elements. Although most nuclear receptors are regulated by ligand binding no cognate ligands(s) have been definitively identified for any ROR protein. There are three different ROR proteins: RORa, RORb and RORg. An isoform of RORg, called ROR gamma T, is exclusively expressed in lymphocytes. Together, RORa and ROR gamma T are critical transcription factors required for differentiation of naive CD4+ Tcells into Th17 cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

Publications for VPR-66, ROR gamma T and ROR alpha Inhibitor Inhibitor (NBP2-29335)(2)

Publications using NBP2-29335

• Hsu, AY;Wang, T;Syahirah, R;Liu, S;Li, K;Zhang, W;Wang, J;Cao, Z;Tian, S;Matosevic, S;Staiger, CJ;Wan, J;Deng, Q; Rora Regulates Neutrophil Migration and Activation in Zebrafish Frontiers in immunology [PMID: 35309302]
• Dang Y, Ling S, Ma J et al. Bavachalcone Enhances RORalpha Expression, Controls Bmal1 Circadian Transcription, and Depresses Cellular Senescence in Human Endothelial Cells. Evid Based Complement Alternat Med 2015-01-01 [PMID: 26199639] (WB, Human)

FAQs for VPR-66, ROR gamma T and ROR alpha Inhibitor Inhibitor (NBP2-29335). (Showing 1 - 1 of 1 FAQs).

1. Do you have data for concentration for mouse injection (in-vivo)?
   • We don't have any data on in vivo use of the inhibitor, nor know of any publications. However, we would recommend a starting dose of 20 mg/kg and going up in dosage from there. You would need to empirically determine the suitability of the inhibitor for your mouse model system as well as optimize the dosage. The compound can be stored long term at -80.