Vitamin D3 Antibody (685503) [Alexa Fluor® 488]

Human monocyte-derived dendritic cells were stained with Mouse Anti-Vitamin D3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC6566G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: All-Multi
• Applications: Flow
• Clone: 685503
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Vitamin D3 Antibody (685503) [Alexa Fluor® 488] Summary

• Immunogen: 25-Hydroxyvitamin D₃
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Vitamin D3 Antibody (685503) [Alexa Fluor® 488]

• Calcifediol
• Calciol
• Calcitriol
• Cholecalciferol
• VD3
• Vitamin D3

Background

25-hydroxy-vitamin D₃ (25(OH)D₃) is synthesized in the liver and is the primary circulating form of vitamin D.  Its blood concentration, which reflects 25(OH)D₃ produced by exposure to ultraviolet B, as well as dietary and vitamin D supplementation, is felt to be the best indicator of vitamin D status.  25(OH)D₃ is metabolized to 1 alpha ,25(OH)₂D₃ in the  proximal tubular cells of the kidney by the enzyme 1 alpha -hydroxylase.   The vitamin D structure is similar to that of classic steroid hormones, such as estradiol, cortisol, and aldosterone in that they have the same root cyclopentanoperhydrophenanthrene ring structure.  It has been shown that the active steroid hormone 1 alpha ,25(OH)₂D₃ is essential for life in higher animals.  Besides playing important roles in calcium homeostasis and bone mineral metabolism, it is now known to play a role in cellular differentiation, inhibition of cell growth, immune regulation and the prevention of neoplastic transformation. The active form of vitamin D₃, 1 alpha ,25(OH)₂D₃, acts both through its cellular receptor, the vitamin D receptor (VDR), and through other extrarenal targets in an autocrine and paracrine manner where 1 alpha -hydroxylase is present.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.