Rat Vitamin B12 ELISA Kit (Colorimetric)

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma
Standard Curve Range
0.312 pg/ml-20 pg/ml
Sensitivity
0.299 pg/ml

Order Details

Rat Vitamin B12 ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of rat VB12. No significant cross-reactivity or interference between rat VB12 and analogues was observed.
Standard Curve Range
0.312 pg/ml-20 pg/ml
Sensitivity
0.299 pg/ml
Assay Type
Direct ELISA
Inter-Assay
CV%<10%
Intra-Assay
CV%<8%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.

Kit Components

Components
  1. TMB Substrate
  2. Wash Buffer (25 x concentrate)
  3. Biotin-conjugate Diluent
  4. Biotin-conjugate (10 x concentrate)
  5. Adhesive Strip (For 96 wells)
  6. Assay plate
  7. Stop Solution
  8. Standard
  9. Sample Diluent
  10. HRP-avidin (100 x concentrate)
  11. HRP-avidin Diluent
  12. Instruction manual

Notes

Please contact our Technical Services with any questions regarding species reactivity

Background

This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for VB12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated VB12. A competitive inhibition reaction is launched between VB12 (Standards or samples) and biotin-conjugated VB12 with the pre-coated VB12 antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of VB12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Array NBP2-60198

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