Plant Vitamin B12 ELISA Kit (Colorimetric)

Product Discontinued

Plant Vitamin B12 ELISA Kit (Colorimetric) Summary

• Specificity: This assay has high sensitivity and excellent specificity for detection of plant VB12. No significant cross-reactivity or interference between plant VB12 and analogues was observed.
• Standard Curve Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: 14.967 pg/ml
• Assay Type: Competitive ELISA
• Inter-Assay: CV% < 10%
• Intra-Assay: CV% < 8%
• Sample Volume: 50-100ul
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Reactivity Notes

Please contact our Technical Services with any questions regarding species reactivity

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Background

This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for VB12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated VB12. A competitive inhibition reaction is launched between VB12 (Standards or samples) and biotin-conjugated VB12 with the pre-coated VB12 antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of VB12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Vitamin B12 ELISA Kit (NBP2-60197). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.