Human Vitamin B12 ELISA Kit (Colorimetric)

Images

 
ELISA: Human Vitamin B12 ELISA Kit (Colorimetric) [NBP2-60196] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma
Standard Curve Range
1.56 pg/ml-100 pg/ml
Sensitivity
1.498 pg/ml

Order Details

Human Vitamin B12 ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of human VB12. No significant cross-reactivity or interference between human VB12 and analogues was observed.
Standard Curve Range
1.56 pg/ml-100 pg/ml
Sensitivity
1.498 pg/ml
Assay Type
Competitive ELISA
Inter-Assay
CV% < 10%
Intra-Assay
CV% < 8%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Publications
Read Publication using NBP2-60196.

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. Stop Solution
  2. Sample Diluent
  3. Standard
  4. Wash Buffer (25 x concentrate)
  5. TMB Substrate
  6. Adhesive Strip (For 96 wells)
  7. Biotin-conjugate (10 x concentrate)
  8. Biotin-conjugate Diluent
  9. Assay plate
  10. Instruction manual
  11. HRP-avidin Diluent
  12. HRP-avidin (100 x concentrate)

Notes

Please contact our Technical Services with any questions regarding species reactivity

Background

This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for VB12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated VB12. A competitive inhibition reaction is launched between VB12 (Standards or samples) and biotin-conjugated VB12 with the pre-coated VB12 antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of VB12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Vitamin B12 ELISA Kit (NBP2-60196)(1)

We have publications tested in 1 application: ELISA.


Filter By Application
ELISA
(1)
All Applications
Filter By Species
All Species

Reviews for Vitamin B12 ELISA Kit (NBP2-60196) (0)

There are no reviews for Vitamin B12 ELISA Kit (NBP2-60196). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Vitamin B12 ELISA Kit (NBP2-60196) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Vitamin B12 Products

Array NBP2-60196

Research Areas for Vitamin B12 ELISA Kit (NBP2-60196)

Find related products by research area.

Blogs on Vitamin B12

There are no specific blogs for Vitamin B12, but you can read our latest blog posts.
Coronavirus Brochure

Contact Information

Product PDFs

Review this Product

Be the first to review our Human Vitamin B12 ELISA Kit (Colorimetric) and receive a gift card or discount.