Human Vitamin B12 ELISA Kit (Colorimetric)


ELISA: Human Vitamin B12 ELISA Kit (Colorimetric) [NBP2-60196] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma
Standard Curve Range
1.56 pg/ml-100 pg/ml
1.498 pg/ml

Order Details

Human Vitamin B12 ELISA Kit (Colorimetric) Summary

This assay has high sensitivity and excellent specificity for detection of human VB12. No significant cross-reactivity or interference between human VB12 and analogues was observed.
Standard Curve Range
1.56 pg/ml-100 pg/ml
1.498 pg/ml
Assay Type
Competitive ELISA
CV% < 10%
CV% < 8%
Sample Volume
Kit Type
ELISA Kit (Colorimetric)


Read Publication using NBP2-60196.

Packaging, Storage & Formulations

Store at 4°C.

Kit Components

  1. Stop Solution
  2. Sample Diluent
  3. Standard
  4. Wash Buffer (25 x concentrate)
  5. TMB Substrate
  6. Adhesive Strip (For 96 wells)
  7. Biotin-conjugate (10 x concentrate)
  8. Biotin-conjugate Diluent
  9. Assay plate
  10. Instruction manual
  11. HRP-avidin Diluent
  12. HRP-avidin (100 x concentrate)


Please contact our Technical Services with any questions regarding species reactivity


This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for VB12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated VB12. A competitive inhibition reaction is launched between VB12 (Standards or samples) and biotin-conjugated VB12 with the pre-coated VB12 antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of VB12 bound in the initial step. The color development is stopped and the intensity of the color is measured.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Vitamin B12 ELISA Kit (NBP2-60196)(1)

We have publications tested in 1 application: ELISA.

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