Mouse Vitamin A ELISA Kit (Colorimetric)

Mouse Vitamin A ELISA Kit (Colorimetric) [NBP2-60192] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum and plasma
• Standard Curve Range: 187.5 pg/mL - 3000 pg/mL
• Sensitivity: 140.159 pg/mL

Mouse Vitamin A ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse VA. No significant cross-reactivity or interference between mouse VA and analogues was observed.
• Standard Curve Range: 187.5 pg/mL - 3000 pg/mL
• Sensitivity: 140.159 pg/mL
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VA. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated VA. The competitive inhibition reaction is launched between with HRP-conjugated VA and VA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of VA in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Vitamin A ELISA Kit (NBP2-60192)(1)

Publication using NBP2-60192

• Zhou Y, Zhou J, Zhang Y et al. Changes in Intestinal Microbiota Are Associated with Islet Function in a Mouse Model of Dietary Vitamin A Deficiency Journal of Diabetes Research 2020-01-22 [PMID: 32064275]

FAQs for Vitamin A ELISA Kit (NBP2-60192). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.