Human Vitamin A ELISA Kit (Colorimetric)

Human Vitamin A ELISA Kit (Colorimetric) [NBP2-60191] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum and plasma
• Standard Curve Range: 0.625 ng/mL - 10 ng/mL
• Sensitivity: 0.467 ng/mL

Human Vitamin A ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of human VA. No significant cross-reactivity or interference between human VA and analogues was observed.
• Standard Curve Range: 0.625 ng/mL - 10 ng/mL
• Sensitivity: 0.467 ng/mL
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 10
• Intra-Assay: %CV < 8
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VA. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated VA. The competitive inhibition reaction is launched between with HRP-conjugated VA and VA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of VA in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Vitamin A ELISA Kit (NBP2-60191)(2)

Publications using NBP2-60191

• Miguel-Camacho A, Garcia-Guillen K, Aguirre-Garza M et al. Effect of coagulation time on the concentration of epitheliotrophic components of autologous serum. Research Square 2020-10-08
• Carazo A, MacAkovA K, MatouSovA K et al. Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity Nutrients 2021-05-18 [PMID: 34069881] (ELISA)

FAQs for Vitamin A ELISA Kit (NBP2-60191). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.