Description | VisUBlock(TM) Mouse on Mouse Blocking Reagent effectively blocks endogenous IgG, which enables staining of mouse tissue
with mouse IgG primary antibodies and eliminates unwanted background staining. Endogenous IgG poses a significant
issue when using mouse IgG primary antibodies on mouse tissue: anti-mouse secondary detection antibodies will not only
bind to mouse IgG primary antibodies, but also to endogenous tissue IgG. Therefore, if endogenous IgG is not blocked prior
to incubation with secondary detection antibodies, intense background staining occurs which can mask specific staining
generated by the primary antibody. 1.0 mL of concentrate is enough to stain 100-250 tissue sections when diluted. |
Application Notes | Blocking reagent for use with primary antibodies from mouse host and mouse tissue. Key Benefits: Reduces background staining Enables staining of mouse tissue with mouse IgG primary antibodies |
Storage | Store at 4C. Do not freeze. |