Vector® TrueVIEW™ Autofluorescence Quenching Kit

Vector(R) TrueVIEW(TM) Autofluorescence Quenching Kit [SP-8500-15-NB] - Human tonsil (FFPE). Section stained for AE1/AE3 using fluorescein label (green). Treated with TrueVIEW and mounted with VECTASHIELD Vibrance Antifade Mounting Medium with DAPI (nuclei blue).

Vector(R) TrueVIEW(TM) Autofluorescence Quenching Kit [SP-8500-15-NB]

Vector® TrueVIEW™ Autofluorescence Quenching Kit Summary

• Description: The TrueVIEW Autofluorescence Quenching Kit with DAPI provides a novel way to diminish unwanted autofluorescence from non-lipofuscin sources and dramatically improve signal-to-noise ratio. It removes unwanted autofluorescence in tissue sections due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin. The quenching action of the kit reagents provides a clear, unambiguous, “true view” localization of the target antigen.
  
  Features:
• Effective on problematic tissues such as kidney and spleen
• Easy-to-use, one-step method
• Compatible with a wide selection of fluorophores
• Compatible with standard epifluorescence and confocal laser microscopes
• Mounting medium contains DAPI for counterstaining in one step


One kit is sufficient to treat approximately 100 to 150 tissue sections.
• Kit Type: Autofluorescence Quenching Kit

Applications/Dilutions

• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

Tissue autofluorescence often occurs with aldehyde fixation or from inherent native tissue components (collagen, elastin, and red blood cells). The extent and intensity of autofluorescence background frequently makes it difficult or impossible to distinguish specific signals in immunofluorescence applications. Most methods for reduction of tissue autofluorescence act primarily on lipofuscin granules, and are not broadly effective against the most common sources of autofluorescence targeted by Vector(R) TrueVIEW(TM) Quencher. The Vector(R) TrueVIEW(TM) Autofluorescence Quenching Kit provides a novel way to diminish unwanted autofluorescence from non-lipofuscin sources and dramatically improve signal-to-noise ratio. The quenching action of the kit reagents provides a clear, unambiguous, true view localization of the target antigen. Current methods for reducing autofluorescence primarily include home brew concoctions such as sodium borohydride and other ink-based products. These methods are essentially ineffective against aldehyde induced autofluorescence. In contrast, Vector(R) TrueVIEW(TM) reagent binds to hydrophilic compounds and effectively quenches endogenous autofluorescence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.