Vector® TrueVIEW™ Autofluorescence Quenching Kit

Immunohistochemistry: Vector(R) TrueVIEW(TM) Autofluorescence Quenching Kit [SP-8400-NB] - Pancreas (FFPE), antigen retrieved with Antigen Unmasking Solution, stained with Guinea Pig x Insulin (green) and Mouse x END (red), followed by FL-Goat x Guinea Pig IgG + Dylight(R) 594-Horse x Mouse IgG. Mounted in VECTASHIELD(R) HardSet(TM) Mounting Medium with DAPI.No TrueVIEW(TM) Quencher => 20x objective, red channel exposed 600ms, green channel 600ms.With TrueVIEW(TM) Quencher => 20x objective, red channel exposed 600ms, green channel exposed 600ms.

Immunohistochemistry: Vector(R) TrueVIEW(TM) Autofluorescence Quenching Kit [SP-8400-NB] - Spleen (FFPE), antigen retrieved with Antigen Unmasking Solution, stained using Mouse Anti-CD20 (red) and Rabbit Anti-Ki67 (green), followed by VectaFluor(TM) Duet Kit, and mounted in VECTASHIELD(R) HardSet(TM) Mounting Medium with DAPI. Left: No TrueVIEW(TM) Quencher treatment - note interfereing autofluorescence. Right: Treated with TrueVIEW(TM) Autofluorescence Quencher.No TrueVIEW(TM) Quencher => 20x objective, red channel exposed 200ms, green channel 200ms.With TrueVIEW(TM) Quencher => 20x objective, red channel exposed 200ms, green channel exposed 800ms.

• Applications: Func, IHC

Vector® TrueVIEW™ Autofluorescence Quenching Kit Summary

• Description: Vector(R) TrueVIEW(TM) Autofluorescence Quenching Kit provides a novel way to remove unwanted fluorescence in tissue sections due to aldehyde fixation, red-blood cells, and structural elements such as collagen and elastin. This unique formulation binds and effectively quenches the autofluorescent elements in even the most problematic tissues, such as kidney, spleen and pancreas. The use of Vector(R) TrueVIEW(TM) leads to significant enhancement in overall signal-to-noise in most immunofluorescence assays.
• Kit Type: Autofluorescence Quenching Kit

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • In-situ Hybridization
• Application Notes: One kit is sufficient to treat approximately 100 to 150 tissue sections..

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

Tissue autofluorescence often occurs with aldehyde fixation or from inherent native tissue components (collagen, elastin, and red blood cells). The extent and intensity of autofluorescence background frequently makes it difficult or impossible to distinguish specific signals in immunofluorescence applications. Most methods for reduction of tissue autofluorescence act primarily on lipofuscin granules, and are not broadly effective against the most common sources of autofluorescence targeted by Vector(R) TrueVIEW(TM) Quencher. The Vector(R) TrueVIEW(TM) Autofluorescence Quenching Kit provides a novel way to diminish unwanted autofluorescence from non-lipofuscin sources and dramatically improve signal-to-noise ratio. The quenching action of the kit reagents provides a clear, unambiguous, true view localization of the target antigen. Current methods for reducing autofluorescence primarily include home brew concoctions such as sodium borohydride and other ink-based products. These methods are essentially ineffective against aldehyde induced autofluorescence. In contrast, Vector(R) TrueVIEW(TM) reagent binds to hydrophilic compounds and effectively quenches endogenous autofluorescence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.