VECTASTAIN® Universal Quick anti-mouse/rabbit/goat IgG HRP Immunodetection Kit

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Immunohistochemistry: VECTASTAIN(R) Universal Quick anti-mouse/rabbit/goat IgG HRP Immunodetection Kit [PK-7800-NB] - Prostate: Desmin (m), VECTASTAIN Universal Quick Kit, ImmPACT DAB (brown) substrate. Hematoxylin QS ...read more

Product Details

Summary
Reactivity Mu, Gt, RbSpecies Glossary
Applications ICC/IF, IHC

Order Details

VECTASTAIN® Universal Quick anti-mouse/rabbit/goat IgG HRP Immunodetection Kit Summary

Description
Features:
  • Rapid protocol: Staining in less than 20 minutes following primary antibody incubation
  • Working solutions can be used immediately after dilution.
  • High sensitivity, low background.
  • Biotinylated, Universal Pan-Specific secondary antibody recognizes mouse, rabbit, and goat primary antibodies as well as those from related species such as rat, bovine, and sheep.*
  • Available in Ready-To-Use (R.T.U) format: Prediluted, stabilized working solutions of VECTASTAIN® Universal Quick Kit reagents provide the same high sensitivity and low background as the concentrated VECTASTAIN® Universal Kit.

  • *Do not use the pan-specific secondary antibody to stain rat, mouse or other rodent, rabbit, goat, bovine, or sheep tissue due to potential reactivity with endogenous IgG.
    Kit Type
    HRP Immunodetection Kit

    Applications/Dilutions

    Dilutions
    • Immunocytochemistry/ Immunofluorescence
    • Immunohistochemistry
    Application Notes
    Sufficient reagents to stain approximately 250-500 sections..

    Packaging, Storage & Formulations

    Storage
    Store at 4C. Do not freeze.

    Kit Components

    Components
    1. 50 ml 2.5% normal horse serum
    2. 50 ml prediluted biotinylated universal pan-specific (anti-mouse/rabbit/goat IgG) secondary antibody
    3. 50 ml prediluted peroxidase-streptavidin complex

    Notes

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    Background

    With more than 50,000 citations to its credit, the VECTASTAIN(R) ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.
    Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN(R) ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    ICC/IF Video Protocol

    FAQs for VECTASTAIN(R) Universal Quick anti-mouse/rabbit/goat IgG Kit (PK-7800-NB). (Showing 1 - 1 of 1 FAQ).

    1. what is the different between PK-7800-NB and 8800
      • PK-7800-NB is ready to use, and PK-8800-NB requires reagent preparation. With both you can stain up to 500 sections.

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