VECTASTAIN® Universal Quick anti-mouse/rabbit/goat IgG HRP Immunodetection Kit Summary
Description |
Features:
Rapid protocol: Staining in less than 20 minutes following primary antibody incubation
Working solutions can be used immediately after dilution.
High sensitivity, low background.
Biotinylated, Universal Pan-Specific secondary antibody recognizes mouse, rabbit, and goat primary antibodies as well as those from related species such as rat, bovine, and sheep.*
Available in Ready-To-Use (R.T.U) format: Prediluted, stabilized working solutions of VECTASTAIN® Universal Quick Kit reagents provide the same high sensitivity and low background as the concentrated VECTASTAIN® Universal Kit. *Do not use the pan-specific secondary antibody to stain rat, mouse or other rodent, rabbit, goat, bovine, or sheep tissue due to potential reactivity with endogenous IgG. |
Kit Type |
HRP Immunodetection Kit |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry
|
Application Notes |
Sufficient reagents to stain approximately 250-500 sections.. |
Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze. |
Kit Components
Components
|
- 50 ml 2.5% normal horse serum
- 50 ml prediluted biotinylated universal pan-specific (anti-mouse/rabbit/goat IgG) secondary antibody
- 50 ml prediluted peroxidase-streptavidin complex
|
Notes
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
Background
With more than 50,000 citations to its credit, the VECTASTAIN(R) ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.
Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN(R) ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for VECTASTAIN(R) Universal Quick anti-mouse/rabbit/goat IgG Kit (PK-7800-NB). (Showing 1 - 1 of 1 FAQ).
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what is the different between PK-7800-NB and 8800
- PK-7800-NB is ready to use, and PK-8800-NB requires reagent preparation. With both you can stain up to 500 sections.
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