VECTASTAIN® ELITE® ABC HRP Detection Kit

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Immunohistochemistry-Paraffin: VECTASTAIN(R) ELITE(R) ABC HRP Detection Kit [PK-6100-NB] - Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), ...read more
Immunohistochemistry-Paraffin: VECTASTAIN(R) ELITE(R) ABC HRP Detection Kit [PK-6100-NB] - Paraffin embedded human prostate tissue stained using rabbit anti-prostate specific antigen labeled with the ProtOn Biotin ...read more
Immunohistochemistry-Paraffin: VECTASTAIN(R) ELITE(R) ABC HRP Detection Kit [PK-6100-NB] - Breast Carcinoma (triple label): Estrogen Receptor (m); VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), ...read more

Product Details

Summary
Applications WB, ELISA, ICC/IF, IHC, ISH, ESPOT

Order Details

VECTASTAIN® ELITE® ABC HRP Detection Kit Summary

Description
Features:
  • Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target
  • Highest sensitivity, low background: The VECTASTAIN® Elite® ABC system is the most sensitive avidin/biotin-based peroxidase system. The Elite® ABC series is approximately 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background.
  • Available without ("Standard" kit) or with biotinylated species-specific or universal secondary antibodies
  • Available in ready-to-use formats: Prediluted, stabilized working solutions of Elite® ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN® Elite® ABC Kit reagents.
  • Kit Type
    HRP Detection Kit

    Applications/Dilutions

    Dilutions
    • ELISA
    • ELISPOT
    • Immunocytochemistry/Immunofluorescence
    • Immunohistochemistry
    • In-situ Hybridization
    • Western Blot
    Application Notes
    Kit contains sufficeint reagents to stain approximately 500-1000 tissue sections.

    Packaging, Storage & Formulations

    Storage
    Store at 4C. Do not freeze.

    Kit Components

    Components
    1. Biotinylated affinity-purified anti-Immunoglobulin - 1 ml
    2. Blocking Serum (Normal Serum) - 3 ml
    3. Reagent A (Avidin DH) - 2 ml
    4. Reagent B (Biotinylated Horseradish Peroxidase H) - 2 ml

    Notes

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    Background

    With more than 50,000 citations to its credit, the VECTASTAIN(R) ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.
    Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN(R) ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    WB Video Protocol
    ICC/IF Video Protocol

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