VECTASTAIN® ABC AP Staining Kit (Colorimetric)

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Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      AK-5000NB
    • Availability
      Product Discontinued

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VECTASTAIN® ABC AP Staining Kit (Colorimetric) Summary

Description
Features:
Alkaline phosphatase enzyme detection system
Avidin/biotin technology
High sensitivity/low background
Low cost
Preferred for tissues that have high endogenous peroxidase activity
Offers additional substrate choices

Used as a second label in multiple antigen labeling applications
Specificity
Reagent A and B when mixed form the ABC-AP complex.
Kit is sufficient to stain approximately 1000-2000 sections or fifty 100 cm2 blots.
Kit Type
AP Staining Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • In-situ Hybridization
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at 4°C.

Kit Components

Components
  1. 2 ml Reagent A (Avidin DH)
  2. 2 ml Reagent B (Biotinylated AP)

Background

With more than 50,000 citations to its credit, the VECTASTAIN ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.
The sensitivity of the VECTASTAIN ABC-AP system is comparable to that of the peroxidase VECTASTAIN Elite ABC system. The VECTASTAIN ABC-AP Kits may be preferred for tissues that have high endogenous peroxidase activity. The system also offers additional substrate color choices.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Additional VECTASTAIN(R) ABC Products

Array AK-5000NB

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