VECTASTAIN® ABC anti-mouse/rabbit AP Immunodetection Kit

Immunohistochemistry: VECTASTAIN(R) ABC anti-mouse/rabbit AP Immunodetection Kit [AK-5200-NB] - Tumor (double label): p53 protein (m), VECTASTAIN ABC-AP Kit, Vector Red substrate (red); Pan-Cytokeratin (m), VECTASTAIN Elite ABC Kit, Vector SG Substrate (blue/gray).

Immunohistochemistry: VECTASTAIN(R) ABC anti-mouse/rabbit AP Immunodetection Kit [AK-5200-NB] - Tonsil (double label): Cyclin A (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue); CD20 (m), VECTASTAIN Universal Elite ABC Kit, Vector NovaRED substrate (red).

Immunohistochemistry: VECTASTAIN(R) ABC anti-mouse/rabbit AP Immunodetection Kit [AK-5200-NB] - Tonsil (double label): Cyclin A (m), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red); CD3 (m), VECTASTAIN Universal Elite ABC Kit, Vector DAB substrate (brown).

Immunohistochemistry: VECTASTAIN(R) ABC anti-mouse/rabbit AP Immunodetection Kit [AK-5200-NB] - Endometrium (double label): Progesterone Receptor (rm), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue); CD34 (m), VECTASTAIN Universal Elite ABC Kit, DAB substrate (brown).

Immunohistochemistry: VECTASTAIN(R) ABC anti-mouse/rabbit AP Immunodetection Kit [AK-5200-NB] - Mesothelioma (double label): M2A Antigen (m), VECTASTAIN Universal Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue).

Immunohistochemistry: VECTASTAIN(R) ABC anti-mouse/rabbit AP Immunodetection Kit [AK-5200-NB] - Tumor (double label): CD34 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue AP substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red).

Immunohistochemistry: VECTASTAIN(R) ABC anti-mouse/rabbit AP Immunodetection Kit [AK-5200-NB] - Colon (double label): CD34 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue); CD20 (m), ImmPRESS Universal Reagent, Vector NovaRED substrate (red).

Immunohistochemistry: VECTASTAIN(R) ABC anti-mouse/rabbit AP Immunodetection Kit [AK-5200-NB] - Colon (double label): M2A antigen (m), VECTASTAIN ABC-AP Kit (Universal), Vector Blue (blue). CD20 (m), ImmPRESS Reagent (HRP, Universal), Vector VIP (purple).

Immunohistochemistry: VECTASTAIN(R) ABC anti-mouse/rabbit AP Immunodetection Kit [AK-5200-NB] - Colon Carcinoma (double label): S100 (rp), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red); Cytokeratin 8/18 (m), VECTASTAIN Universal Elite ABC Kit, Vector SG substrate (blue/gray).

Immunohistochemistry: VECTASTAIN(R) ABC anti-mouse/rabbit AP Immunodetection Kit [AK-5200-NB] - Breast Carcinoma (double label): Estrogen Receptor (m), ImmPRESS Universal Reagent, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Universal ABC-AP kit, Vector Blue substrate (blue).

Immunohistochemistry: VECTASTAIN(R) ABC anti-mouse/rabbit AP Immunodetection Kit [AK-5200-NB] - Breast Carcinoma (double label): Estrogen Receptor (m), ImmPRESS Universal Reagent, Vector NovaRED substrate (red); Cytokeratin 8/18 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue)

Immunohistochemistry: VECTASTAIN(R) ABC anti-mouse/rabbit AP Immunodetection Kit [AK-5200-NB] - Breast Carcinoma (double label): Estrogen Receptor (m), ImmPRESS Universal Reagent, Vector DAB substrate (brown); Multi-Cytokeratin (m), VECTASTAIN Universal ABC-AP Kit, Vector Red substrate (red)

Immunohistochemistry: VECTASTAIN(R) ABC anti-mouse/rabbit AP Immunodetection Kit [AK-5200-NB] - Colon (double label): M2A Antigen (m), ImmPRESS Universal Reagent, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue)

Immunohistochemistry: VECTASTAIN(R) ABC anti-mouse/rabbit AP Immunodetection Kit [AK-5200-NB] - Tonsil (double label): CD3 (m), VECTASTAIN Universal ABC-AP Kit, Vector Blue substrate (blue); CD20 (m), ImmPRESS Universal Reagent, Vector NovaRED substrate (red).

Immunohistochemistry: VECTASTAIN(R) ABC anti-mouse/rabbit AP Immunodetection Kit [AK-5200-NB] - Lymph Node (double label): Ki67 (m), VECTASTAIN Universal ABC-AP Kit, Vector Red Substrate (red); Multi-cytokeratin (m), VECTASTAIN Universal Elite ABC Kit, DAB substrate (brown).

• Reactivity: Mu, Rb
• Applications: WB, ELISA, Func, ICC/IF, IHC, ELISA

VECTASTAIN® ABC anti-mouse/rabbit AP Immunodetection Kit Summary

• Description: Features:
  
• Alkaline phosphatase enzyme detection system
• Avidin/biotin technology
• High sensitivity/low background
• Low cost
• Preferred for tissues that have high endogenous peroxidase activity
• Offers additional substrate choices
• Used as a second label in multiple antigen labeling applications
• Kit Type: AP Immunodetection Kit

Applications/Dilutions

• Dilutions:
  • ELISA
  • Enzyme-linked Immunosorbent Spot
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • In-situ Hybridization
  • Western Blot
• Application Notes: These kits are sufficient to stain approximately 1000-2000 sections or fifty 100 cm^2 blots..

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

With more than 50,000 citations to its credit, the VECTASTAIN ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory. The sensitivity of the VECTASTAIN ABC-AP system is comparable to that of the peroxidase VECTASTAIN Elite ABC system. The VECTASTAIN ABC-AP Kits may be preferred for tissues that have high endogenous peroxidase activity. The system also offers additional substrate color choices.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.