VECTASTAIN® ABC anti-Mouse IgM HRP Immunodetection Kit

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ICC/IF, IHC, ELISA (-), ESPOT

Order Details

VECTASTAIN® ABC anti-Mouse IgM HRP Immunodetection Kit Summary

Description
Features:
  • Orignial avidin/biotin ABC complex formation
  • Good sensitivity/Low background
  • Available without ("Standard") or with biotinylated species-specific secondary antibody
  • Kit Type
    HRP Immunodetection Kit

    Applications/Dilutions

    Dilutions
    • ELISPOT
    • Immunocytochemistry/Immunofluorescence
    • Immunohistochemistry
    • Western Blot
    Application Notes
    Kit contains sufficient reagents to stain approximately 1000-2000 sections.

    Packaging, Storage & Formulations

    Storage
    Store at 4C. Do not freeze.

    Kit Components

    Components
    1. 1 ml biotinylated goat anti-mouse IgM
    2. 2 ml Reagent A
    3. 2 ml Reagent B
    4. 3 ml normal goat serum

    Notes

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    Background

    With more than 50,000 citations to its credit, the VECTASTAIN(R) ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.
    Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN(R) ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    WB Video Protocol
    ICC/IF Video Protocol

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