VECTASTAIN® ABC anti-Mouse IgG AP Immunodetection Kit

Immunohistochemistry: Vectastain(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB] - Tumor (double label): p53 (m), VECTASTAIN ABC-AP Kit, Vector Blue substrate (blue); Multi-Cytokeratin (m), VECTASTAIN Elite ABC Kit, AEC substrate (red).

Immunohistochemistry: Vectastain(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB] - Prostate (double label): Cytokeratin (m), VECTASTAIN ABC-AP Kit, Vector Blue (blue) substrate. CD34, VECTASTAIN ABC-AP Kit, Vector Red (red) substrate.

Immunohistochemistry: Vectastain(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB] - Tumor (double label): Cytokeratin, VECTASTAIN Elite ABC Kit, Vector NovaRED (red) substrate. CD34, VECTASTAIN ABC-AP Kit, BCIP/NBT (blue) substrate.

Immunohistochemistry: Vectastain(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB] - Breast Carcinoma (triple label): Estrogen Receptor (m), VECTASTAIN Elite ABC Kit, Vector NovaRED substrate (red); CD34 (m), VECTASTAIN ABC-AP Kit, Vector Blue substrate (blue); Cytokeratin 8/18 (m), VECTASTAIN Elite ABC Kit, DAB substrate (brown).

VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB]

VECTASTAIN(R) ABC anti-Mouse IgG AP Immunodetection Kit [AK-5002-NB]

• Reactivity: Mu
• Applications: WB, ELISA, Func, ICC/IF, IHC, ELISA

VECTASTAIN® ABC anti-Mouse IgG AP Immunodetection Kit Summary

• Description: Features:
• Alkaline phosphatase enzyme detection system
• Avidin/biotin technology
• High sensitivity/low background
• Preferred for tissues that have high endogenous peroxidase activity
• Offers additional substrate choices
• Used as a second label in multiple antigen labeling applications
• Kit Type: AP Immunodetection Kit

Applications/Dilutions

• Dilutions:
  • ELISA
  • Enzyme-linked Immunosorbent Spot
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • In-situ Hybridization
  • Western Blot
• Application Notes: This kit is sufficient to stain approximately 1000-2000 sections or fifty 100 cm^2 blots..

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

With more than 50,000 citations to its credit, the VECTASTAIN ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory. The sensitivity of the VECTASTAI ABC-AP system is comparable to that of the peroxidase VECTASTAIN Elite ABC system. The VECTASTAIN ABC-AP Kits may be preferred for tissues that have high endogenous peroxidase activity. The system also offers additional substrate color choices

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.