VECTASHIELD® HardSet™ Antifade Mounting Medium

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Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB] - VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - C6 rat glioma cells: Antibody to mitochondria ...read more
Immunohistochemistry: VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight 594 Goat Anti-Rabbit ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium [H-1400-NB] - VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - C6 rat glioma cells: Antibody to mitochondria ...read more
Immunohistochemistry: VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - Prostate Specific Antigen (rp), fluorescein labeled using the ProtOn Fluorescein Labeling Kit. Propidium Iodide counterstain (red). ...read more
Immunohistochemistry: VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - Prostate Specific Antigen (rp), fluorescein labeled using the ProtOn Fluorescein Labeling Kit. Propidium Iodide counterstain (red). ...read more
Immunohistochemistry: VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Fluorescein Avidin DCS (green). Mounted in VECTASHIELD HardSet Mounting ...read more
Immunohistochemistry: VECTASHIELD(R) Hardset(TM) Antifade Mounting Medium [H-1400-NB] - Alzheimer's disease mouse brain model - double label: glial cells stained with Iba1 (rp) followed by DyLight 594 Goat Anti-Rabbit ...read more

Product Details

Summary
Applications ICC/IF, ISH, CIMG, SR Microscopy

Order Details

VECTASHIELD® HardSet™ Antifade Mounting Medium Summary

Description
VECTASHIELD Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.

VECTASHIELD HardSet Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved. VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins.

Features:
  • Inhibits photobleaching of fluorescent dyes and fluorescent proteins
  • Ideal refractive index (1.45)
  • Ready-to-use
  • No warming necessary
  • Can be stored without sealing for long term analysis
  • Hardening formulation
  • No counterstain


  • Applications/Dilutions

    Application Notes
    Refractive index is 1.46.
    Reviewed Applications
    Read 2 Reviews rated 5
    using
    H-1400-NB in the following application:

    Packaging, Storage & Formulations

    Storage
    Store at 4C in the dark.

    Notes

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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    Reviews for VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium (H-1400-NB) (2) 52

    Average Rating: 5
    (Based on 2 reviews)

    Reviews using H-1400-NB:
    Filter by Applications
    ICC/IF
    (1)
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    reviewed by:
    Hailong Song
    ICC/IF 01/15/2020
    View

    Summary

    ApplicationImmunocytochemistry/Immunofluorescence
    LotZF1029
     
    reviewed by:
    Hailong Song
    01/15/2020
    View

    Summary

    ApplicationSerum
    LotZF1029

    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    ICC/IF Video Protocol

    FAQs for VECTASHIELD(R) HardSet(TM) Antifade Mounting Medium (H-1400-NB) (0)

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    Recent Reviews

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    Hailong Song
    01/15/2020
    Application: ICC/IF
    Species:

     
    Hailong Song
    01/15/2020
    Application:
    Species: