VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin

Images

 
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonic fibroblast stained with mouse anti-tubulin followed by fluorescein horse ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Cytokeratin (m) detected with DyLight 488 Anti-Mouse IgG, ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Cytokeratin (m) detected with DyLight 488 Anti-Mouse IgG, ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Tubulin (m) detected with DyLight 488 Anti-Mouse IgG and ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Clathrin (m) detected with DyLight 488 Anti-Mouse IgG, ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Cytokeratin (m) detected with DyLight 488 Anti-Mouse IgG, ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Integrin (m) detected with DyLight 488 Anti-Mouse IgG, ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Clathrin (m) detected with DyLight 488 Anti-Mouse IgG, ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Clathrin (m) detected with DyLight 488 Anti-Mouse IgG, ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Cytokeratin (m) detected with DyLight 488 Anti-Mouse IgG, ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Tubulin (m) detected with DyLight 488 Anti-Mouse IgG and ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonal fibroblasts: Anti-Integrin (m) detected with DyLight 488 Anti-Mouse IgG, ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonic fibroblast stained with mouse anti-tubulin followed by fluorescein horse ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with Phalloidin [H-1600-NB] - Mouse embryonic fibroblast stained with mouse anti-tubulin followed by fluorescein horse ...read more

Product Details

Summary
Applications ICC/IF, ISH, CIMG

Order Details

VECTASHIELD® HardSet™ Antifade Mounting Medium with Phalloidin Summary

Description
VECTASHIELD Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.

Features:

  • Inhibits photobleaching of fluorescent dyes and fluorescent proteins
  • Ideal refractive index (1.45)
  • Ready-to-use - from the refrigerator to the benchtop
  • No warming necessary
  • Can be stored without sealing for long term analysis
  • Hardening formulation
  • TRITC-Phalloidin counterstain

  • VECTASHIELD HardSet Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.

    VECTASHIELD HardSet Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. This special formulation of VECTASHIELD HardSet Mounting Medium contains TRITC-phalloidin. Phalloidin is a bicyclic heptapeptide that specifically binds at the interface between the F-actin subunits. Fluorescent derivatives of phalloidin are used to stain actin filaments.

    TRITC (tetramethylrhodamine) is excited at 544 nm and emits at 572 nm, producing an orange-red fluorescence.

    Applications/Dilutions

    Dilutions
    • Cellular Imaging
    • Immunocytochemistry/Immunofluorescence
    • In-situ Hybridization
    Application Notes
    The refractive index is 1.46.

    Packaging, Storage & Formulations

    Storage
    Store at 4C in the dark.

    Notes

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    ICC/IF Video Protocol

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