VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI

Images

 
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 ...read more
Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein label ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 594 labeled secondary antibody. Mounted in ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 594 labeled secondary antibody. Mounted in ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 549 labeled secondary antibody. Mounted in ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 488 ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 594 ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 549 ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 488 labeled secondary antibody. Mounted in ...read more
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Astrocytes: Stained for GFAP and detected with Dylight 549 labeled secondary antibody. Mounted in ...read more
Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Texas Red Avidin DCS (red). Mounted in VECTASHIELD HardSet ...read more
Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein ...read more
Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein ...read more
Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Prostate: Prostate Specific Antigen (rp) labeled with fluorescein using the ProtOn Fluorescein Labeling Kit. Fluorescein ...read more
Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Retina from mice, perfused with DyLight 594-LEL. The retina were sectioned and mounted in Vectashield HardSet+DAPI. ...read more
Immunohistochemistry: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Colon: Desmin (m), Biotinylated Horse anti-mouse IgG, Texas Red Avidin DCS (red). Mounted in VECTASHIELD HardSet ...read more
Cellular Imaging: VECTASHIELD(R) Hard Set(TM) Antifade Mounting Medium with DAPI [H-1500-NB] - Dorsal root ganglia cells (neurons and satellite glia) - double label. BIII tubulin, DyLight 488 labeled secondary antibody ...read more

Product Details

Summary
Applications ICC/IF, ISH, CIMG

Order Details

VECTASHIELD® HardSet™ Antifade Mounting Medium with DAPI Summary

Description
VECTASHIELD Hardset Antifade Mounting Medium with DAPI is a unique, stable formula for preserving fluorescence. VECTASHIELD Hardset Mounting Medium with DAPI prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.

Features:

  • Inhibits photobleaching of fluorescent dyes and fluorescent proteins
  • Ideal refractive index (1.45)
  • Ready-to-use
  • No warming necessary
  • Can be stored without sealing for long term analysis
  • Hardening formulation
  • DAPI counterstain


  • VECTASHIELD HardSet Mounting Medium preserves fluorescence and hardens after coverslipping. After approximately 15 minutes at room temperature, the coverslip will become immobilized, and optimal antifade ability and refractive index will be achieved.

    VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD Mounting Medium without DAPI. DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.

    Applications/Dilutions

    Dilutions
    • Cellular Imaging
    • Immunocytochemistry/Immunofluorescence
    • In-situ Hybridization
    Application Notes
    Refractive index is 1.46.

    NOTE: This product has a six month expiration date.

    Packaging, Storage & Formulations

    Storage
    Store at 4C in the dark.

    Notes

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    ICC/IF Video Protocol

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