VECTASHIELD® Antifade Mounting Medium with Propidium Iodide

Images

 
Immunocytochemistry/ Immunofluorescence: VECTASHIELD(R) Antifade Mounting Medium with Propidium Iodide [H-1300-NB] - FastTag Fluorescein labeled pUC1.77 detected with Biotinylated Anti-Fluorescein and Fluorescein Avidin ...read more
In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with Propidium Iodide [H-1300-NB] - Mounting Medium with Propidium Iodide [H-1300-NB] - Human chromosome 6 paint detected with FITC-labeled probe (yellow) ...read more

Product Details

Summary
Applications ICC/IF, ISH, CIMG

Order Details

VECTASHIELD® Antifade Mounting Medium with Propidium Iodide Summary

Description
VECTASHIELD Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.

Features:

  • Inhibits photobleaching of fluorescent dyes and fluorescent proteins
  • Ideal refractive index (1.45)
  • Ready-to-use
  • No warming necessary
  • Can be stored without sealing for long term analysis
  • Non-hardening formulation
  • Propidium iodide (PI) counterstain


  • The original VECTASHIELD Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4C, protected from light.

    VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. The original VECTASHIELD Mounting Medium is available with or without the counterstain PI (propidium iodide). PI is a reddish nuclear counterstain with a broad excitation range at around 530 nm and emission at about 615 nm.

    Applications/Dilutions

    Dilutions
    • Cellular Imaging
    • Immunocytochemistry/Immunofluorescence
    • In-situ Hybridization
    Application Notes
    Refractive index is 1.45.

    Packaging, Storage & Formulations

    Storage
    Store at 4C in the dark.

    Notes

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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    Product General Protocols

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    Video Protocols

    ICC/IF Video Protocol

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