VECTASHIELD® Antifade Mounting Medium with DAPI

Images

 
In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - FastTag Biotin-labeled pUC1.77 detected with Texas Red Avidin DCS, Biotinylated Anti-Avidin and Texas Red Avidin DCS, and ...read more
In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - FastTag FL-labeled pUC1.77 on interphase nuclei detected with Alkaline Phosphatase Anti-Fluorescein followed by Vector Red ...read more
In-situ Hybridization: VECTASHIELD(R) Antifade Mounting Medium with DAPI [H-1200-NB] - Single copy DNA probe to the Downs region of chromosome 21 using FITC-labeled antibody (green) and mounted in VECTASHIELD Mounting ...read more

Product Details

Summary
Applications Func, ICC/IF, IHC, MS

Order Details

VECTASHIELD® Antifade Mounting Medium with DAPI Summary

Description
VECTASHIELD Antifade Mounting Medium is a unique, stable formula for preserving fluorescence. VECTASHIELD Mounting Medium prevents rapid photobleaching of fluorescent proteins and fluorescent dyes.

Features:

  • Inhibits photobleaching of fluorescent dyes and fluorescent proteins
  • Ideal refractive index (1.45)
  • Ready-to-use
  • No warming necessary
  • Can be stored without sealing for long term analysis
  • Non-hardening formulation
  • No counterstain


  • The original VECTASHIELD Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4C, protected from light.

    VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins.

    The original VECTASHIELD Mounting Medium does not solidify, but remains a liquid on the slide and can be stored without sealing. If desired, coverslips can be sealed around the perimeter with nail polish or a plastic sealant. Mounted slides should be stored at 4 degrees C, protected from light. VECTASHIELD Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins.

    Both the VECTASHIELD HardSet and the original VECTASHIELD Mounting Media are available with or without the counterstain DAPI (4,6-diamidino-2-phenylindole). The DAPI concentration can be modified by mixing with the corresponding VECTASHIELD Mounting Medium without DAPI. DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.

    Applications/Dilutions

    Dilutions
    • Cellular Imaging
    • Immunocytochemistry/ Immunofluorescence
    • Immunohistochemistry
    • Immunohistochemistry-Frozen
    • In-situ Hybridization
    Application Notes
    The refractive index for VECTASHIELD Mounting Medium is 1.45. Use in IHC-Fr reported in scientific publication (PMID: 32452384).
    Reviewed Applications
    Read 1 Review rated 5
    using
    H-1200-NB in the following applications:

    Publications
    Read Publications using
    H-1200-NB in the following applications:

    Packaging, Storage & Formulations

    Storage
    Store at 4C in the dark.

    Notes

    This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

    Publications for VECTASHIELD(R) Antifade Mounting Medium with DAPI (H-1200-NB)(7)

    We have publications tested in 1 confirmed species: Invertebrate.

    We have publications tested in 2 applications: ICC/IF, IF/IHC.


    Filter By Application
    ICC/IF
    (1)
    IF/IHC
    (1)
    All Applications
    Filter By Species
    Invertebrate
    (1)
    All Species
    Showing Publications 1 - 7 of 7.
    Publications using H-1200-NB Applications Species
    Trotta MC, Petrillo F, Gesualdo C et al. Effects of the Calix[4]arene Derivative Compound OTX008 on High Glucose-Stimulated ARPE-19 Cells: Focus on Galectin-1/TGF-beta/EMT Pathway Molecules (Basel, Switzerland) 2022-07-26 [PMID: 35897964]
    Widen JC, Tholen M, Yim JJ et al. AND-gate contrast agents for enhanced fluorescence-guided surgery Nature Biomedical Engineering 2020-09-28 [PMID: 32989286]
    Weir K, Dupre C, van Giesen L et al. A molecular filter for the cnidarian stinging response Elife 2020-05-26 [PMID: 32452384] (IF/IHC, Invertebrate) IF/IHC Invertebrate
    Perl AL, O'Connor CM, Fa P et al. Protein phosphatase 2A controls ongoing DNA replication by binding to and regulating cell division cycle 45 (CDC45) J. Biol. Chem. [PMID: 31562245] (ICC/IF) ICC/IF
    Han J, Robinet P, Ritchey B et al. Confirmation of Ath26 locus on chromosome 17 and identification of Cyp4f13 as an atherosclerosis modifying gene Atherosclerosis 2019-05-09 [PMID: 31102955]
    BBLT L, Nabatanzi R, Mausser E et al. Diversity of Vaginal Microbiota Affects Epithelial Barrier Permeability Among African Pregnant Women Research Square 2021-02-22
    Thiha P, Higashihori N, Kano S, Moriyama K Histone methyltransferase SET domain bifurcated 1 negatively regulates parathyroid hormone/parathyroid hormone-related peptide receptor to control chondrocyte proliferation in Meckel’s cartilage Archives of Oral Biology 2021-08-01 [PMID: 34521010]

    Review for VECTASHIELD(R) Antifade Mounting Medium with DAPI (H-1200-NB) (1) 51

    Average Rating: 5
    (Based on 1 review)

    Reviews using H-1200-NB:
    Filter by Applications
    All Applications
    Filter by Species
    All Species
    Images Ratings Applications Species Date Details
      5
    reviewed by:
    Verified Customer
    11/06/2018
    View

    Summary

    LotZE0815

    Product General Protocols

    Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

    Video Protocols

    ICC/IF Video Protocol

    FAQs for VECTASHIELD(R) Antifade Mounting Medium with DAPI (H-1200-NB) (0)

    There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

    Additional VECTASHIELD(R) Antifade Mounting Medium with DAPI Products

    Blogs on VECTASHIELD(R) Antifade Mounting Medium with DAPI

    There are no specific blogs for VECTASHIELD(R) Antifade Mounting Medium with DAPI, but you can read our latest blog posts.

    Contact Information

    Product PDFs

    Recent Reviews

    5
    5
    1
    4
    0
    3
    0
    2
    0
    1
    0

     
    Verified Customer
    11/06/2018
    Application:
    Species: