Vaccinia Virus Antibody (TV46)

Product Discontinued

Vaccinia Virus Antibody (TV46) Summary

• Immunogen: Tissue / cell preparation: Vaccinia Virus (vaccine strain)
• Localization: Whole virus
• Specificity: Vaccinia Virus (TV46)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000

Reactivity Notes

Cross-reacts with Vaccinia Virus.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Vaccinia Virus Antibody (TV46)

• A27L
• Orthopoxvirus

Background

Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Vaccinia Virus Antibody (NB110-17317)(1)

Publication using NB110-17317

• Da Costa TP Advances in the Diagnosis and Epidemiology of Orthopoxvirus Infection in Non-Domestic Animals and its Reservoir Hosts in the United Kingdom Thesis 1905-07-13 (IF/IHC)