v6.5 Mouse embryonic stem cells Whole Cell Lysate

Western Blot: v6.5 Mouse embryonic stem cells Whole Cell Lysate [NBP1-97433] - Western blot analysis of Beta Actin (NB600-532) using v6.5 Mouse embryonic stem cells Lysate (NBP1-97433).

SDS-Page: v6.5 Mouse embryonic stem cells Whole Cell Lysate [NBP1-97433] - Ponceau Stain: Staining of v6.5 Mouse embryonic stem cells Lysate using NBP1-97433.

Product Discontinued

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Application Notes: This lysate is prepared as a positive control for immunoprecipitation or separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT loading buffer or reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS. No reducing agents or loading buffer added.
• Preservative: No Preservative
• Concentration: 3.0 mg/ml

Lysate Details for Array

• Type: Cell
• Tissue: Embryo
• Subcellular Fraction: Whole

Background

The v6.5 mouse embryonic stem cell line was developed in the laboratory of Dr. Rudolph Jaenisch of the Whitehead Institute. The cell line was established from cells derived from the inner cell mass (ICM) of a 3.5 day old mouse embryo from a C57BL/6 X 129/sv cross and has been used successfully in producing germline transmitting chimeras. The v6.5 mouse ES cell lines is the parent strain from which the DGCR8 -/- knockout cell line (NBA1-19349) is derived.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.