This lysate is prepared as a positive control for immunoprecipitation or separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT loading buffer or reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS. No reducing agents or loading buffer added.
Lysate Details for v6.5 Mouse embryonic stem cells
The v6.5 mouse embryonic stem cell line was developed in the laboratory of Dr. Rudolph Jaenisch of the Whitehead Institute. The cell line was established from cells derived from the inner cell mass (ICM) of a 3.5 day old mouse embryo from a C57BL/6 X 129/sv cross and has been used successfully in producing germline transmitting chimeras. The v6.5 mouse ES cell lines is the parent strain from which the DGCR8 -/- knockout cell line (NBA1-19349) is derived.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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