Urea Assay Kit (Colorimetric)

Urea Assay Kit (Colorimetric) [KA1652] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Product Discontinued

Urea Assay Kit (Colorimetric) Summary

• Description: Quality control test: Standard Curve Urea Assay Kit is a quantitative colorimetric urea determination.
• Standard Curve Range: 0.08 to 100 mg/dL (13 uM to 17 mM)
• Sensitivity: 0.08 mg/dL (13 uM)
• Assay Type: Colorimetric
• Sample Volume: 5 uL
• Kit Type: Assay Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • Functional
  • Quantification

Reactivity Notes

Reacts with Resident species.

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

Urea, primarily produced in the liver and secreted by the kidneys, is the major end product of protein catabolism in animals and is the primary vehicle for removal of toxic ammonia from the body. Urea determination is very useful for the assesment of kidney function of research subjects. In general, increased urea levels are associated with nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal diseases, e.g., congestive heart failure, liver diseases and diabetes. Decreased levels indicate acute hepatic insufficiency or may result from over-vigorous parenteral fluid therapy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Urea Kit (KA1652)(19)

Publications using KA1652

• ALTamimi J, AlFaris N, Alshammari G et al. Ellagic acid protects against diabetic nephropathy in rats by regulating the transcription and activity of Nrf2 Journal of Functional Foods 2021-04-01 (QFN, Rat)
• Benoit C, Patrick B, Reiner C et al. Setup of human liver-chips integrating 3D models, microwells and a standardized microfluidic platform as proof-of-concept study to support drug evaluation. Biomater Biosyst. 2022-08-15 [PMID: 36824483]
• Wenke M, Hagen M Preoperative Function Assessment of Ex Vivo Kidneys with Supervised Machine Learning Based on Blood and Urine Markers Measured during Normothermic Machine Perfusion. Biomedicines. 2022-11-28 [PMID: 36551812]
• Zoe O, Sabine E, Beatrix W et al. Microdosing as a Potential Tool to Enhance Clinical Development of Novel Antibiotics: A Tissue and Plasma PK Feasibility Study with Ciprofloxacin. Clin Pharmacokinet. 2022-01-07 [PMID: 34997559]
• Alejo C, Gabriela M, Julieta M et al. Enhanced ammonia detoxification to urea in hepatocytes transduced with human aquaporin-8 gene. Biotechnol Bioeng. 2021-07-28 [PMID: 34292591]
• Iuliia P, Morgan S, Mark D et al. Inhibition of neprilysin with sacubitril without RAS blockage aggravates renal disease in Dahl SS rats. Ren Fail. 2021-02-04 [PMID: 33541194]
• Hafiz F, Bohye K, Muhammad K et al. Real-time monitoring of liver fibrosis through embedded sensors in a microphysiological system. Nano Converg. 2021-02-02 [PMID: 33528697]
• K Takeishi, A Collin de, Y Wang, K Handa, J Guzman-Lep, K Matsubara, K Morita, S Jang, N Haep, RM Florentino, F Yuan, K Fukumitsu, K Tobita, W Sun, J Franks, ER Delgado, EM Shapiro, NA Fraunhoffe, AW Duncan, H Yagi, T Mashimo, IJ Fox, A Soto-Gutie Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells Cell Rep, 2020-06-02;31(9):107711. 2020-06-02 [PMID: 32492423]
• W G, D P, N D et al. Is It Possible to Store Spotted Wolffish (Anarhichas Minor) Sperm by Refrigeration? Fish Physiol Biochem. 2020-06-02 [PMID: 32488550]
• Iuliia P, Mark D, Rebecca F et al. The Differential Effects of Low Dose Sacubitril and/or Valsartan on Renal Disease in Salt-Sensitive Hypertension. Am J Physiol Renal Physiol. 2020-05-28 [PMID: 32463726]
• Alejo C, Maria A, Raul M et al. Data of ureagenesis from ammonia, glutamine and alanine, and mitochondrial aquaporin-8 expression in thioacetamide-treated hepatocytes. Data Brief. 2020-04-30 [PMID: 32395591]
• Zaramella P, Munari F, Stocchero M et al. Innate immunity ascertained from blood and tracheal aspirates of preterm newborn provides new clues for assessing bronchopulmonary dysplasia. PLoS One. 2019-09-04 [PMID: 31483807]
• Zhang K, Zhang L, Liu W et al. In Vitro Expansion of Primary Human Hepatocytes with Efficient Liver Repopulation Capacity. Cell Stem Cell 2018-11-08 [PMID: 30416071]
• Starokozhko V, Hemmingsen M, Larsen L et al. Differentiation of human-induced pluripotent stem cell under flow conditions to mature hepatocytes for liver tissue engineering. J Tissue Eng Regen Med. 2018-04-06 [PMID: 29499107]
• YS Hwang, JH Shin, JP Yang, BK Jung, SH Lee, EH Shin Characteristics of Infection Immunity Regulated byToxoplasma gondiito Maintain Chronic Infection in the Brain Front Immunol, 2018-02-05;9(0):158. 2018-02-05 [PMID: 29459868]
• Cozzolino AM, Noce V, Battistelli C et al. Modulating the Substrate Stiffness to Manipulate Differentiation of Resident Liver Stem Cells and to Improve the Differentiation State of Hepatocytes. Stem Cells International. 2015-10-13 [PMID: 27057172]
• Sahin E, Haubenwallner S, Kuttke M et al. Macrophage PTEN regulates expression and secretion of arginase I modulating innate and adaptive immune responses. J Immunol. 2014-08-15 [PMID: 25015834]
• Ebert T, Hopf LM, Wurst U et al. Circulating adipocyte fatty acid binding protein is increased in chronic and acute renal dysfunction. Nutr Metab Cardiovasc Dis. 2014-04-02 [PMID: 24813306]
• Sin YY, Ballantyne LL, Mukherjee K et al. Inducible Arginase 1 Deficiency in Mice Leads to Hyperargininemia and Altered Amino Acid Metabolism. PLoS One. 2013-11-04 [PMID: 24224027]