Mouse Universal Protein Whole Tissue Lysate (Adult Whole Normal)

• Reactivity: Mu
• Applications: WB
• Concentration: 5.0 mg/ml

Mouse Universal Protein Whole Tissue Lysate (Adult Whole Normal) Summary

• Description: Mouse Universal Protein Lysate
  Prepared from several male and female Balb/C mice whole bodies without fur. This protein lysate serves as standard for Western Blot control of human protein studies.
  
  Contents
  2 x 0.5 mg per package
• Specificity: Universal Protein Lysate: Mouse Normal Tissues

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
• Concentration: 5.0 mg/ml

Lysate Details for Array

• Type: Tissue
• Life Stage: Adult
• Tissue: Universal
• Protein State: Native
• Tissue Condition: Normal
• Subcellular Fraction: Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for Universal Protein Lysate (NB820-89439). (Showing 1 - 2 of 2 FAQ).

1. Do you know the size of the inhibitors in this cocktail? I am going to denature the proteins, so if they are susceptible to denaturation I can forget about them? Or should I use a spin column to remove low molecular weight compounds?
   • These inhibitors are all < 10 kDa in size, so if you don't want to buy a large quantity, you can definitely use a spin column with a 10 kDa molecular weight cutoff to remove them. I feel that this is the better strategy. This is because, without protease inhibitors, there will be both denaturation and degradation of the lysate protein. If you are performing protease studies, it is better to start with an intact full-length protein.
2.  I want to test different proteases on a variety of substrates, and thought universal whole tissue lysate as a good choice. Unfortunately it contains a cocktail of protease inhibitors. Is it possible to get some without these inhibitors?
   • We can make the protein without the protease inhibitors, but the minimum quantity for this custom product will be 5 mg. If you do want to obtain this format, please do let us know and we could work on the pricing and its lead time for this custom order.