U-2 OS Whole Cell Lysate

• Reactivity: Hu
• Applications: WB, IP, PAGE
• Concentration: 5.0 mg/ml

U-2 OS Whole Cell Lysate Summary

• Specificity: U-2 OS (human osterogenic sarcoma) whole cell lysate (non-denatured).

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • SDS-Page
  • Western Blot
• Application Notes: Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression, and mass spec. analysis.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: HEPES (pH7.9), KCl, Sucrose, Sodium deoxycholate, and NP-40 (without denaturing agents).
• Preservative: No Preservative
• Concentration: 5.0 mg/ml

Lysate Details for Array

• Type: Cell
• Tissue: Bone/Cartilage
• Protein State: Native
• Subcellular Fraction: Whole

Background

U2OS is a human bone osteosarcoma from a human 15 year old Caucasian female expressing wild type p53 and lacking p16. U2OS also has a ZFN modification creating a GFP-NUP98 transgene expressed from the endogenous NUP98 gene locus. U2OS cells were established in 1964, the original cells were taken from a moderately differentiated sarcoma of the tibia. U2OS cells are positive for insulin-like growth factor I (IGF-I) and insulin-like growth factor II (IGF II) receptors and express a number of antigens, including blood type A, Rh+, HLA A2, Aw30, B12, Bw35, and B40(+/-).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for U-2 OS Lysate (NBP2-13171). (Showing 1 - 1 of 1 FAQ).

1. What is the Biosafety level of NBP2-13171? Can you provide the disease screening certificate?
   • Our U-2 OS cell lysate product NBP2-13171 is Biosafety level 1. We unfortunately have not performed any disease screening on this product, so we do not have a certificate to provide.