Tumor-derived Exosome Capture (Biofluids) and RNA Extraction Kit

Product Discontinued

Tumor-derived Exosome Capture (Biofluids) and RNA Extraction Kit Summary

• Description: Features
  
• Fast and user friendly protocol
• Small starting amount of sample (less than 1 ml)
• Better RNA yield than similar products
• Kit includes Exosome Standards as control
• RNA extraction from tumor-derived exosome population captured with immunobeads
• Kit Type: Extraction Kit

Reactivity Notes

Human biological fluids including plasma, serum, urine and cell culture media.

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Background

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases. Exosomes shuttle functional RNA molecules in the target cell. Increasing evidence suggests a role for exosome-derived miRNAs in the development and/or progression of specific human diseases. Pathogenic miRNAs might be exploited as novel therapeutic targets or disease biomarkers in complex diseases, including cancer. In fact, miRNAs seem to play critical roles as transcriptional and post-transcriptional regulators of epigenetic mechanisms and cell processes and have been linked to the etiology, progression and prognosis of cancer. Similar miRNA expression patterns between tumor tissue samples and circulating exosomes have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.