Tryptophan ELISA Kit (Colorimetric)

ELISA: Tryptophan ELISA Kit [HRP] [KA1916] - Typical calibration curve

• Applications: ELISA, NULL
• Suitable Sample Type: Plasma, Serum, Urine
• Standard Curve Range: 0.73 to 250 ug/mL
• Sensitivity: 1.2 ug/mL

Tryptophan ELISA Kit (Colorimetric) Summary

• Description: Tryptophan ELISA Kit is an enzyme immunoassay for the quantitative determination of tryptophan.
• Standard Curve Range: 0.73 to 250 ug/mL
• Sensitivity: 1.2 ug/mL
• Assay Type: Quantification
• Inter-Assay: CV% < 11.7%
• Intra-Assay: CV% < 11%
• Spike Recovery: Urine: 106%, Serum: 95%
• Sample Volume: 25 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Tryptophan ELISA Kit (KA1916)(7)

Publications using KA1916

• Chengcao Sun, Youqiong Ye, Zhi Tan, Yuan Liu, Yajuan Li, Wei Hu, Ke Liang, Sergey D. Egranov, Lisa Angela Huang, Zhao Zhang, Yaohua Zhang, Jun Yao, Tina K. Nguyen, Zilong Zhao, Andrew Wu, Jeffrey R. Marks, Abigail S. Caudle, Aysegul A. Sahin, Jianjun Gao, Seth T. Gammon, David Piwnica-Worms, Jian Hu, Paul J. Chiao, Dihua Yu, Mien-Chie Hung, Michael A. Curran, George A. Calin, Haoqiang Ying, Leng Han, Chunru Lin, Liuqing Yang Tumor-associated nonmyelinating Schwann cell–expressed PVT1 promotes pancreatic cancer kynurenine pathway and tumor immune exclusion Science Advances 2023-02-01 [PMID: 36724291]
• Sun L, Zhang Y, Yang T et al. IGFBP2 Drives Regulatory T Cell Differentiation through STAT3/IDO Signaling Pathway in Pancreatic Cancer Journal of personalized medicine 2022-12-03 [PMID: 36556226] (ELISA)
  
  Details:
  Tissue supernatants
• Yuki T, Junko N, Dai H et al. CNS serotonin content mediating food deprivation-enhanced learning is regulated by hemolymph tryptophan concentration and autophagic flux in the pond snail. Nutr Neurosci. 2022-02-14 [PMID: 35156560]
• Allison B, Lindsey R, Adam N et al. Tryptophan metabolism is dysregulated in individuals with Fanconi anemia. Blood Adv. 2021-01-12 [PMID: 33570643]
• Jiao H, Yan Z, Ma Q et al. Influence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus. Neuropsychiatr Dis Treat. 2019-01-09 [PMID: 30587994]
• Liu R, Merola J, Manes TD et al. Interferon-gamma converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1. JCI Insight. 2018-03-08 [PMID: 29515027] (ELISA, Human)
• Martin-Gronert MS, Stocker CJ, Wargent ET et al. 5-HT2A and 5-HT2C receptors as hypothalamic targets of developmental programming in male rats. Dis Model Mech 2016-01-14 [PMID: 26769798]

FAQs for Tryptophan ELISA Kit (KA1916). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.