Rat Triiodothyronine ELISA Kit (Colorimetric)

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ELISA: Rat Triiodothyronine ELISA Kit (Colorimetric) [NBP2-60185] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma, cell culture supernates, tissue homogenates
Standard Curve Range
0.5 ng/ml-8 ng/ml
Sensitivity
0.5 ng/ml

Order Details

Rat Triiodothyronine ELISA Kit (Colorimetric) Summary

Specificity
This assay has high sensitivity and excellent specificity for detection of rat T3. No significant cross-reactivity or interference between rat T3 and analogues was observed.
Standard Curve Range
0.5 ng/ml-8 ng/ml
Sensitivity
0.5 ng/ml
Assay Type
Competitive ELISA
Inter-Assay
CV%<15%
Intra-Assay
CV%<15%
Sample Volume
50-100ul
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.

Kit Components

Components
  1. Conjugate
  2. Instruction manual
  3. Assay plate
  4. Adhesive Strip (For 96 wells)
  5. Substrate A
  6. Substrate B
  7. Wash Buffer (20 x concentrate)
  8. Standard
  9. Stop Solution

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Rat Triiodothyronine ELISA Kit (Colorimetric)

  • liothyronine; T3

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T3. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated T3. A competitive inhibition reaction is launched between T3 (Standards or samples) and Biotin-conjugated T3 with the pre-coated antibody specific for T3. The more amount of T3 in samples, the less antibody bound by Biotin-conjugated T3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T3 in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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