Chicken Triiodothyronine ELISA Kit (Colorimetric)

• Reactivity: Ch
• Applications: ELISA
• Suitable Sample Type: serum, plasma, tissue homogenates
• Standard Curve Range: 0.5 - 8 ng/mL
• Sensitivity: 0.5 ng/mL

Chicken Triiodothyronine ELISA Kit (Colorimetric) Summary

• Specificity: This assay has high sensitivity and excellent specificity for detection of chicken T3. No significant cross-reactivity or interference between chicken T3 and analogues was observed.
• Standard Curve Range: 0.5 - 8 ng/mL
• Sensitivity: 0.5 ng/mL
• Assay Type: Competitive ELISA
• Inter-Assay: %CV < 15
• Intra-Assay: %CV < 15
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Chicken Triiodothyronine ELISA Kit (Colorimetric)

• liothyronine; T3

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T3. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T3. A competitive inhibition reaction is launched between T3 (Standards or samples) and Biotin-conjugated T3 with the pre-coated antibody specific for T3. The more amount of T3 in samples, the less antibody bound by Biotin-conjugated T3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T3 in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.