Human Trachea Whole Tissue Lysate (Adult Whole Normal)

• Reactivity: Hu
• Applications: WB
• Concentration: 5.0 mg/ml

Human Trachea Whole Tissue Lysate (Adult Whole Normal) Summary

• Description: We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.
• Specificity: Total Protein - Human Adult Normal Tissue: Trachea

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
• Concentration: 5.0 mg/ml

Lysate Details for Array

• Type: Tissue
• Life Stage: Adult
• Tissue: Respiratory
• Protein State: Native
• Tissue Condition: Normal
• Subcellular Fraction: Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Trachea Lysate (NB820-59241)(1)

Publication using NB820-59241

• Kashem MA, Li L, Yuan XY et al. Toll-like Interleukin -1 Receptor Regulator (TILRR) Protein, a Major Modulator of Inflammation, is Expressed in Normal Human and Macaque Tissues and PBMCs Journal of Inflammation Research 2022-05-12 [PMID: 35592073] (Western Blot, Immunocytochemistry/ Immunofluorescence)