Rat Toxoplasmosis (TOXO) Antibody (IgG) ELISA Kit (Colorimetric) Summary
Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of rat anti-toxoplasmosis(TOXO) antibody (IgG). No significant cross-reactivity or interference between rat anti-toxoplasmosis(TOXO) antibody (IgG) and analogues was observed.
Assay Type
Indirect ELISA
Inter-Assay
%CV < 15
Intra-Assay
%CV < 15
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Applications/Dilutions
Dilutions
ELISA
Publications
Read Publication using NBP2-60168 in the following applications:
Storage of components varies. See protocol for specific instructions.
Kit Components
Components
Adhesive Strip (For 96 wells)
Coated assay plate
HRP-conjugate
Instruction manual
Negative Control
Positive Control
Sample Diluent
Stop Solution
Substrate A
Substrate B
Wash Buffer (10 x concentrate)
Background
This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-rat IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of rat anti-toxoplasmosis(TOXO) antibody (IgG) bound in the initial step. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Publications for Toxoplasmosis (TOXO) Antibody (IgG) ELISA Kit (NBP2-60168)(1)
We have publications tested in 1 confirmed species: Rat.
We have publications tested in 1 application: ELISA.
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Product General Protocols
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