Toxoplasma gondii ROP1 Antibody (3D4B2) - BSA Free

Western Blot: Toxoplasma gondii ROP1 Antibody (3D4B2) [NBP2-61906] - Analysis using ROP1 mAb against Toxoplasma gondii rhoptry protein ROP1 (AA: 42-183) recombinant protein. (Expected MW is 41.1 kDa)

Immunocytochemistry/Immunofluorescence: Toxoplasma gondii ROP1 Antibody (3D4B2) [NBP2-61906] - Analysis of SMMC-7721 cells using ROP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry: Toxoplasma gondii ROP1 Antibody (3D4B2) [NBP2-61906] - Analysis of paraffin-embedded ROP1 tissues using bladder cancer mouse mAb with DAB staining.

Western Blot: Toxoplasma gondii ROP1 Antibody (3D4B2) [NBP2-61906] - Analysis using ROP1 mAb against HEK293 (1) and ROP1 (AA: 42-183)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: Toxoplasma gondii ROP1 Antibody (3D4B2) [NBP2-61906] - Analysis of Hela cells using ROP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry-Paraffin: Toxoplasma gondii ROP1 Antibody (3D4B2) [NBP2-61906] - Analysis of paraffin-embedded ROP1 tissues using cervical cancer mouse mAb with DAB staining.

ELISA: Toxoplasma gondii ROP1 Antibody (3D4B2) [NBP2-61906] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Pz
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3D4B2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Toxoplasma gondii ROP1 Antibody (3D4B2) - BSA Free Summary

• Immunogen: Purified recombinant fragment of Toxoplasma gondii rhoptry protein Toxoplasma gondii ROP1 (AA: 42-183) expressed in E. Coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ROP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 42.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Toxoplasma gondii ROP1 Antibody (3D4B2) - BSA Free

• Toxoplasma gondii rhoptry protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ROP1