Toxoplasma Gondii P30 Antibody (P30/3) - BSA Free

Flow Cytometry: Toxoplasma Gondii P30 Antibody (P30/3) [NB100-66486] - analysis of Toxoplasma Gondii P30 in human foreskin fibroblasts using anti-oxoplasma Gondii P30 antibody. Sample was infected with RH Toxoplasma gondii. Image from verified customer review.

• Reactivity: Pr, Pz
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: P30/3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Toxoplasma Gondii P30 Antibody (P30/3) - BSA Free Summary

• Immunogen: Purified T. gondii P30 antigen
• Specificity: Specific for the Toxoplasma gondii P30 membrane protein.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Western Blot 1:100-1:2000
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 26003519)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Toxoplasma Gondii P30 Antibody (P30/3) - BSA Free

• p30
• SAG1 protein
• SAG1

Background

Toxoplasma is a crescent shaped sporozoan that lives as an intracellular parasite in various tissues of many vertebrates and completes its life cycle in a single host. Its life cycle includes two phases called the intestinal (or enteroepithelial) and extraintestinal phases. The intestinal phase produces oocysts and occurs only in cats, wild as well as domesticated. The extraintestinal phase occurs in all infected animals including cats, and produces tachyzoites (actively proliferating trophozoites) and eventually, bradyzoites (slowly growing trophozoites) or zoitocysts. Infection due to Toxoplasma gondii occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy. Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Toxoplasma Gondii P30 Antibody (NB100-66486)(3)

Publications using NB100-66486

• Yanuk JG, Alomar ML, Gonzalez MM et al. A comprehensive analysis of direct and photosensitized attenuation of Toxoplasma gondii tachyzoites. J. Photochem. Photobiol. B, Biol. 2017-10-07 [PMID: 29031212] (WB)
• Baba M, Kitoh K, Takashima Y. Removal of extracellular Toxoplasma gondii tachyzoites from suspended cell culture. Parasitol. Int. 2016-01-21 [PMID: 26806654]
• Baba M, Sato M, Kitoh K, Takashima Y. The distribution pattern of alpha2,3- and alpha2,6-linked sialic acids affects host cell preference in Toxoplasma gondii. Exp. Parasitol. 2015-05-21 [PMID: 26003519] (ICC/IF)

Review for Toxoplasma Gondii P30 Antibody (NB100-66486) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Sara Cohen Flow Human 11/26/2014

Summary

• Application: Flow Cytometry
• Sample Tested: Human Foreskin Fibroblasts
• Species: Human
• Lot: 13/04-11-Tx19-TP3-PE