Toxoplasma Gondii P30 Antibody (P30/3)

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Flow Cytometry: Toxoplasma Gondii P30 Antibody (P30/3) [NB100-66486] - analysis of Toxoplasma Gondii P30 in human foreskin fibroblasts using anti-oxoplasma Gondii P30 antibody. Sample was infected with RH Toxoplasma ...read more

Product Details

Summary
Reactivity Pr, PzSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
P30/3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Toxoplasma Gondii P30 Antibody (P30/3) Summary

Immunogen
Purified T. gondii P30 antigen
Specificity
Specific for the Toxoplasma gondii P30 membrane protein.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 26003519)
Reviewed Applications
Read 1 Review rated 4
using
NB100-66486 in the following applications:

Publications
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Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Toxoplasma Gondii P30 Antibody (P30/3)

  • p30
  • SAG1 protein
  • SAG1

Background

Toxoplasma is a crescent shaped sporozoan that lives as an intracellular parasite in various tissues of many vertebrates and completes its life cycle in a single host. Its life cycle includes two phases called the intestinal (or enteroepithelial) and extraintestinal phases. The intestinal phase produces oocysts and occurs only in cats, wild as well as domesticated. The extraintestinal phase occurs in all infected animals including cats, and produces tachyzoites (actively proliferating trophozoites) and eventually, bradyzoites (slowly growing trophozoites) or zoitocysts. Infection due to Toxoplasma gondii occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy. Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Toxoplasma Gondii P30 Antibody (NB100-66486)(3)

Review for Toxoplasma Gondii P30 Antibody (NB100-66486) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-66486:
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Flow
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Images Ratings Applications Species Date Details
Flow Cytometry Toxoplasma Gondii P30 NB100-66486
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4
reviewed by:
Verified Customer
Flow Human 11/26/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman Foreskin Fibroblasts
SpeciesHuman
Lot13/04-11-Tx19-TP3-PE

Blocking

Blocking Detailsno blocking

Primary Anitbody

Dilution Ratio1:200 in saponin buffer, 30 minutes at 4C

Details

Detection NotesFlow cytometer (BD FACS Canto)
Fixation Details15 min 3% paraformaldehyde fixation at 4C
Permeabilization Detailssaponin buffer (1 wash)
Wash DescriptionFACS buffer (PBS/1% BSA/2 mM EDTA)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Toxoplasma Gondii P30 Antibody (NB100-66486) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-66486AF350
Alexa Fluor 405 NB100-66486AF405
Alexa Fluor 488 NB100-66486AF488
Alexa Fluor 532 NB100-66486AF532
Alexa Fluor 594 NB100-66486AF594
Alexa Fluor 647 NB100-66486AF647
Alexa Fluor 700 NB100-66486AF700
Alexa Fluor 750 NB100-66486AF750
Allophycocyanin NB100-66486APC
Allophycocyanin/Cy7 NB100-66486APCCY7
Biotin NB100-66486B
DyLight 350 NB100-66486UV
DyLight 405 NB100-66486V
DyLight 488 NB100-66486G
DyLight 550 NB100-66486R
DyLight 594 NB100-66486DL594
DyLight 650 NB100-66486C
DyLight 680 NB100-66486FR
DyLight 755 NB100-66486IR
FITC NB100-66486F
HRP NB100-66486H
Janelia Fluor 549 NB100-66486JF549
Janelia Fluor 646 NB100-66486JF646
PE NB100-66486PE
PE/Atto594 NB100-66486PEATT594
PE/Cy5.5 NB100-66486PECY55
PE/Cy7 NB100-66486PECY7
PerCP NB100-66486PCP

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Recent Reviews

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Verified Customer
11/26/2014
Application: Flow
Species: Human