Toxoplasma Gondii ELISA Kit (Colorimetric) Summary
| Description |
Toxoplasma gondii IgA ELISA Kit is a solid phase enzyme immunoassay for the determination of IgA autoantibodies against Toxoplasma gondii. |
| Assay Type |
solid phase enzyme-linked immunosorbent assay |
| Inter-Assay |
CV% < 7.37% |
| Intra-Assay |
CV% < 5% |
| Sample Volume |
100 uL (1:100 prediluted) |
| Kit Type |
ELISA Kit (Colorimetric) |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Toxoplasma gondii IgA ELISA Kit is a solid phase enzyme immunoassay for the qualitative determination of IgA autoantibodies against Toxoplasma gondii. |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
Kit Components
|
Components
|
- 1) r.t.u., ready to use.
- Bag with zipper + desiccant
- Chromogenic substrate TMB r.t.u.
- Cover membrane
- Cut-off control r.t.u.
- Dilution buffer r.t.u.
- ELISA break-away strips coated with the specific antigen
- Negative control r.t.u. 1)
- Peroxidase conjugate (anti-IgA/Px) r.t.u.
- Positive control r.t.u.
- Stop solution r.t.u.
- Wash buffer 25x concentrated
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Toxoplasma Gondii ELISA Kit (Colorimetric)
Background
Toxoplasma is a crescent shaped sporozoan that lives as an intracellular parasite in various tissues of many vertebrates and completes its life cycle in a single host. Its life cycle includes two phases called the intestinal (or enteroepithelial) and extraintestinal phases. The intestinal phase produces oocysts and occurs only in cats, wild as well as domesticated. The extraintestinal phase occurs in all infected animals including cats, and produces tachyzoites (actively proliferating trophozoites) and eventually, bradyzoites (slowly growing trophozoites) or zoitocysts. Infection due to Toxoplasma gondii occurs in pregnant women where a variable degree of immunosuppression may exist or in patients receiving immunosuppressive drug therapy. Toxoplasma infects tissue of the GI tract where an active infection is accompanied by fever and enlargement of the spleen. Symptoms of toxoplasmosis are generally mild but severe infection of lymph nodes may occur. Congenital toxoplasmosis, in which the maternal infection is transmitted during pregnancy, can produce blindness or mental retardation in the newborn.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Toxoplasma Gondii ELISA Kit (KA1462). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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