![Total and Free Cholesterol Assay Kit (Fluorometric) [NBP3-25935] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-25935_total-and-free-cholesterol-assay-kit-fluorometric-8120249432924.png "Total and Free Cholesterol Assay Kit (Fluorometric) [NBP3-25935] - Standard Curve Reference")
| Reactivity | All-NASpecies Glossary |
| Applications | Func |
| Suitable Sample Type | Serum, plasma, animal tissue and cell samples |
| Standard Curve Range | 0.12 - 30 umol/L |
| Sensitivity | 0.12 umol/L |
| Description | Detection instrument: Fluorescence microplate reader (Ex/Em = 535 nm/590 nm) Assay Time: 20 min |
| Standard Curve Range | 0.12 - 30 umol/L |
| Sensitivity | 0.12 umol/L |
| Inter-Assay | %CV < 7.3 |
| Intra-Assay | %CV < 1.7 |
| Spike Recovery | Average recovery rate: 96% |
| Kit Type | Assay Kit (Fluorometric) |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Components |
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![Total and Free Cholesterol Assay Kit (Fluorometric) [NBP3-25935] - Assay Example](http://images.novusbio.com/fullsize/nbp3-25935_total-and-free-cholesterol-assay-kit-fluorometric-81202410594322.png "Total and Free Cholesterol Assay Kit (Fluorometric) [NBP3-25935] - Assay Example")
![Total and Free Cholesterol Assay Kit (Fluorometric) [NBP3-25935] - Assay Example](http://images.novusbio.com/fullsize/nbp3-25935_total-and-free-cholesterol-assay-kit-fluorometric-81202410583825.png "Total and Free Cholesterol Assay Kit (Fluorometric) [NBP3-25935] - Assay Example")