TMEM82 Overexpression Lysate (Native)

Images

 
Western Blot: TMEM82 Overexpression Lysate (Adult Normal) [NBP2-08725] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TMEM82.

Product Details

Summary
Applications WB

Order Details

TMEM82 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_001013663. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens TMEM82, mRNA.
Gene
TMEM82

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for TMEM82

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TMEM82 Overexpression Lysate (Native)

  • transmembrane protein 82

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for TMEM82 Lysate (NBP2-08725) (0)

There are no publications for TMEM82 Lysate (NBP2-08725).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TMEM82 Lysate (NBP2-08725) (0)

There are no reviews for TMEM82 Lysate (NBP2-08725). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TMEM82 Lysate (NBP2-08725) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TMEM82 Overexpression Lysate (Native) Products

Bioinformatics Tool for TMEM82 Lysate (NBP2-08725)

Discover related pathways, diseases and genes to TMEM82 Lysate (NBP2-08725). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on TMEM82

There are no specific blogs for TMEM82, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TMEM82 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol TMEM82

Customers Who Bought This Also Bought

TMEM82 Antibody
NBP2-31814