TMEM81 Overexpression Lysate (Native)

Images

 
Western Blot: TMEM81 Overexpression Lysate (Adult Normal) [NBL1-17106] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TMEM81.

Product Details

Summary
Applications WB

Order Details

TMEM81 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209585 and based on accession number NM_203376. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens transmembrane protein 81 (TMEM81), mRNA.
Gene
TMEM81

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
28.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for TMEM81

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TMEM81 Overexpression Lysate (Native)

  • HC3107
  • KVLA2788
  • MGC75217
  • transmembrane protein 81
  • UNQ2788

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for TMEM81 Lysate (NBL1-17106) (0)

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Additional TMEM81 Overexpression Lysate (Native) Products

TMEM81 NBL1-17106

Bioinformatics Tool for TMEM81 Lysate (NBL1-17106)

Discover related pathways, diseases and genes to TMEM81 Lysate (NBL1-17106). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on TMEM81

There are no specific blogs for TMEM81, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol TMEM81

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