TLR11 Antibody (786404) [Allophycocyanin]

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TLR11 APC-conjugated Monoclonal Antibody (Catalog # IC7640A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Product Discontinued

TLR11 Antibody (786404) [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant mouse TLR11
  Thr209-Gln325
  Accession # Q6R5P0
• Specificity: Detects mouse TLR11 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: TLR11
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TLR11 Antibody (786404) [Allophycocyanin]

• Gm287
• hgnc31753
• TLR11
• Toll like receptor 11

Background

TLR11 is a 110-120 kDa type I transmembrane glycoprotein that belongs to the Toll-like receptor family of proteins that is primarily expressed in intestinal and bladder epithelial cells, dendritic cells, macrophages, astrocytes, neurons and microglia. The 926 amino acid (aa) mouse TLR11 transcript encodes a 30 aa signal sequence, a 691 aa extracellular domain with 10 leucine-rich repeats, a 21 aa transmembrane (TM) domain, and a 184 aa cytoplasmic region with a TIR domain. Within the region used as an immunogen, mouse and rat TLR11 share 86% aa sequence identity. Human TLR11 is a pseudogene that is not expressed. TLR11 resides in the endoplasmic reticulum (ER) where it heterodimerizes with TLR12, and interacts with the 12-TM multispan ER protein UNC93B1, that serves as chaperone and co-factor for TLR11 activity. The receptor recognizes flagelin and profilin from Toxoplasma gondii, plus components of E.coli, S.typhimurium, and promotes IL-12 production by dendritic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR11 Antibody (IC7640A)(1)

Publication using IC7640A

• A Patidar, S Selvaraj, M Chakravart, I Guha, A Bhuniya, S Bera, S Dhar, K Roy, R Baral, D Chattopadh, C Pal, B Saha TLR induced IL-27 plays host-protective role against B16BL6 melanoma in C57BL/6 mice Cytokine, 2022-04-15;154(0):155871. 2022-04-15 [PMID: 35436657] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: TLR11
• Uniprot:
  • Q6R5P0()