Tissue Glue Kit

Immunohistochemistry-Paraffin: Tissue Glue Kit [NBP3-12174] - Formalin fixed paraffin embedded human Tonsil stained with Cytokeratin antibody using Tissue Glue (NBP3-12174)

• Applications: IHC

Tissue Glue Kit Summary

• Kit Type: Kit

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Protocol
  1. This procedure will work for both frozen tissue sections and paraffinembedded tissue sections.
  2. Sections should be mounted on positively-charged glass slides for best results.
  3. For paraffin-embedded sections, the slides should be deparaffinized and rehydrated using standard procedures for deparaffinization.
  4. Place slides into a water bath for five minutes
  5. Remove slides from water bath and carefully remove excess water by wiping around the tissue with an absorbent wipe.
  6. Lay the slides flat onto a level surface and pipette 200 ul of Reagent 1 onto the slides, making sure that the tissue is completely covered with reagent.
  7. Incubate slides for 10 minutes at room temperature.
  8. Drain off Reagent 1 by tipping the slides onto an absorbent towel and allowing reagent to run off the slides into the towel. Do not rinse slides.
  9. Lay the slides flat onto a level surface and pipette 200 ul of Reagent 2 onto the slides, making sure that the tissue is completely covered with reagent.
  10. Incubate slides for 10 minutes at room temperature.
  11. Drain off Reagent 2 by tipping the slides onto an absorbent towel and allow reagent to run off of the slides into the towel. Do not rinse slides.
  12. Lay the slides flat onto a level surface and allow slides to completely dry. Drying time can be shortened by drying slides in a 37 C incubator.
  13. The polymers contained in these reagents prevent drying artifacts, and dried slides can be stored for several days before staining.
  14. At the time of staining, place the slides into an appropriate antigen retrieval solution. The tissue glue will be removed from the slides during the antigen retrieval process.
  15. After antigen retrieval commence immunohistochemical staining.

Packaging, Storage & Formulations

• Storage: Store at 4C.

Background

This product is designed for use with both frozen tissue sections and paraffin-embedded tissue sections for adhereing the tissues firmly onto a glass microscope slide. Tissue Glue uses a two-step process to adhere tissues onto a microscope slide. Tissue Glue is particularly useful for maintaining strong attachment of the tissue to the slide during Heat-induced Epitope Retrieval (HIER) in preparation for immunohistochemical staining. The first reagent is applied to the tissue to form attachment points on the slide and on the tissue. The second reagent contains a glue-like substance that covalently bind to the attachment points, thereby firmly attaching the tissue to the slide. Although Tissue-Glue works with all slides, positively charged slides are recommended in order to achieve the optimal results.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.