Mouse Thyroxine ELISA Kit (Colorimetric)

ELISA: Mouse Thyroxine ELISA Kit (Colorimetric) [NBP2-60162] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: serum, plasma, tissue homogenates
• Standard Curve Range: 20 ng/ml-320 ng/ml
• Sensitivity: 20 ng/ml

Mouse Thyroxine ELISA Kit (Colorimetric) Summary

• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse T4. No significant cross-reactivity or interference between mouse T4 and analogues was observed.
• Standard Curve Range: 20 ng/ml-320 ng/ml
• Sensitivity: 20 ng/ml
• Assay Type: Competitive ELISA
• Inter-Assay: CV% < 15%
• Intra-Assay: CV% < 15%
• Sample Volume: 50-100ul
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

Publications

Read Publication using NBP2-60162 in the following applications: ELISA 1 publication

Packaging, Storage & Formulations

• Storage: Store at 4°C.

Notes

Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Mouse Thyroxine ELISA Kit (Colorimetric)

• T4

Background

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T4. A competitive inhibition reaction is launched between T4 (Standards or samples) and Biotin-conjugated T4 with the pre-coated antibody specific for T4. The more amount of T4 in samples, the less antibody bound by Biotin-conjugated T4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T4 in the sample. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Thyroxine ELISA Kit (NBP2-60162)(1)

Publication using NBP2-60162

• Sudo H, Tsuji AB, Sugyo A Preclinical Evaluation of the Acute Radiotoxicity of the alpha-Emitting Molecular-Targeted Therapeutic Agent 211At-MABG for the Treatment of Malignant Pheochromocytoma in Normal Mice Transl Oncol May 8 2019 [PMID: 31078058] (ELISA, Mouse)