Fish Thyroxine ELISA Kit (Colorimetric)


ELISA: Fish Thyroxine ELISA Kit (Colorimetric) [NBP2-60161] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Reactivity FiSpecies Glossary
Applications ELISA
Suitable Sample Type
serum, plasma and tissue homogenates
Standard Curve Range
20 ng/ml-320 ng/ml
20 ng/ml

Order Details

Fish Thyroxine ELISA Kit (Colorimetric) Summary

This assay has high sensitivity and excellent specificity for detection of fish T4. No significant cross-reactivity or interference between fish T4 and analogues was observed.
Standard Curve Range
20 ng/ml-320 ng/ml
20 ng/ml
Assay Type
Competitive ELISA
Sample Volume
Kit Type
ELISA Kit (Colorimetric)

Packaging, Storage & Formulations

Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date.

Kit Components

  1. HRP-avidin
  2. Instruction manual
  3. Conjugate
  4. Adhesive Strip (For 96 wells)
  5. Assay plate
  6. Stop Solution
  7. Standard
  8. Substrate A
  9. Substrate B
  10. Wash Buffer (20 x concentrate)


Please contact our Technical Services with any questions regarding species reactivity

Alternate Names for Fish Thyroxine ELISA Kit (Colorimetric)

  • T4


This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T4. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T4. A competitive inhibition reaction is launched between T4 (Standards or samples) and Biotin-conjugated T4 with the pre-coated antibody specific for T4. The more amount of T4 in samples, the less antibody bound by Biotin-conjugated T4. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T4 in the sample. The color development is stopped and the intensity of the color is measured.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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