Porcine Thyroxine ELISA Kit (Colorimetric)

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ELISA: Porcine Thyroxine ELISA Kit (Colorimetric) [NBP2-60158] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Porcine Thyroxine ELISA Kit (Colorimetric) [NBP2-60158] - Standard Curve Reference

Product Details

Summary
Reactivity PoSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum and plasma
Standard Curve Range
20 ng/mL - 320 ng/mL
Sensitivity
20 ng/mL

Order Details

Porcine Thyroxine ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of pig T4. No significant cross-reactivity or interference between pig T4 and analogues was observed.
Standard Curve Range
20 ng/mL - 320 ng/mL
Sensitivity
20 ng/mL
Assay Type
Competitive ELISA
Inter-Assay
%CV < 15
Intra-Assay
%CV < 15
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay plate
  3. Conjugate
  4. HRP-avidin
  5. Instruction manual
  6. Standard
  7. Stop Solution
  8. Substrate A
  9. Substrate B
  10. Wash Buffer (20 x concentrate)

Alternate Names for Porcine Thyroxine ELISA Kit (Colorimetric)

  • T4

Background

This assay has high sensitivity and excellent specificity for detection of pig T4. No significant cross-reactivity or interference between pig T4 and analogues was observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Thyroxine ELISA Kit (NBP2-60158). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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