Thyroid Matched Tumor & Normal Tissue Lysate Summary
1 x Human Thyroid tumor tissue lysate (1 mg/ml, 100 ug/vial)
1 x Human Thyroid normal tissue lysate (matched) (1 mg/ml, 100 ug/vial)
Matched Tumor & Normal Tissue Lysate SetDiagnosis: Papillary carcinoma
Stage: III, T3N1M0
Tumor Pathology Data
Gross findings: Tumor size 5 cm, well circumscribed. Cut section firm, white.
Tissue sections show as normal structure of thyroid glands, lined by epithelial cells with coarse, irregular nuclei and papillary structure. The stroma is congestive and infiltrated by numerous lymphocytes, neutrophils, plasma cells, and eosinophils. The lymph nodes show metastasis (2/6).
Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify.
Extraction 1: PBS, pH 7.4; 1 ug/ml Aprotinin; 1 mM NaF
Modified RIPA Buffer: 1 mM EDTA; 1 ug/ml Pepstatin-A; 0.1% SDS; 0.25% Na deoxycholate; 1 ug/ml Leupeptin; 1 mM PMSF; 1 mM Na3VO4
These lysates have not been subjected to denaturing or reducing conditions. This allows the tissue or cell lysate to be used in a variety of applications; to study protein-protein interaction, ligand binding, ELISA, immunoprecipitation, 1D and 2D gel electrophoresis, and Western blotting for the detection of specific protein targets.
For use in 1D and 2D gel electrophoresis, the addition of a denaturing gel loading buffer with reducing agents may be required.
Buffer requirements for performing protein-protein interaction and ligand binding studies can vary significantly from RIPA buffer and may require modifications. In most cases, tissue lysates in RIPA buffer can be used, directly in standard ELISA and immunoprecipitation assays.
These lysates are proteomic discovery tools. Researchers should validate and optimize for individual use.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for Array
Matched Tumor & Normal Tissue
The vial is provided with a 10% overfill. Maximum recovery can be obtained by centrifuging the vial briefly to collect any solution on the cap and tube sides.
This material has tested negative for HbsAg, HIV 1/2, and HCV. Use UNIVERSAL PRECAUTIONS when handling. Human tissue derivatives must be treated as a potentially infectious agent and disposed of appropriately
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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